PXD032170-1

PXD032170 is an original dataset announced via ProteomeXchange.

Title	The translation regulator Zar1l is required to control the timing of meiosis in Xenopus oocytes
Description	In vertebrates, sexual reproduction depends on the precisely temporally controlled translation of mRNAs stockpiled in the oocyte. The RNA-binding proteins Zar1 and Zar2 have been implicated in translational control in oocytes of several vertebrate species, but how they act mechanistically is still incompletely understood. Here, we investigate the function of Zar1l, a so far uncharacterized member of the Zar protein family, in Xenopus laevis oocytes. By combining biochemical assays and mass spectrometry, we reveal that Zar1l is a constituent of a known large ribonucleoparticle containing the translation repressor 4E-T and the central polyadenylation regulator CPEB1. Employing TRIM-Away, we show that depletion of Zar1l from prophase-I arrested oocytes results in premature meiotic maturation upon hormone treatment. We provide evidence that this is based on the precocious expression of the kinase cMos, a key promotor of meiotic resumption. Based on our data, we propose a model according to which degradation of Zar1l results in dissociation of 4E-T from CPEB1, thus weakening translation inhibition imposed by the mRNA 3’UTR of cMos and probably also other M-phase promoting regulators. Thus, our detailed characterization of the function of Zar1l reveals novel mechanistic insights into the regulation of maternal mRNA translation during vertebrate meiosis.
HostingRepository	PRIDE
AnnounceDate	2022-09-22
AnnouncementXML	Submission_2022-09-22_05:46:11.779.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Florian Stengel
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-09 02:39:43	ID requested
⏵ 1	2022-09-22 05:46:12	announced
2	2024-10-22 05:39:46	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Xenopus laevis

Zar1l

Meiosis

Florian Stengel
contact affiliation	Department of Biology, Cellular Proteostasis and Mass Sepectrometry, University of Konstanz
contact email	florian.stengel@uni-konstanz.de
lab head
Florian Stengel
contact affiliation	Universität Konstanz
contact email	florian.stengel@uni-konstanz.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032170
     1. Label: PRIDE project
     2. Name: The translation regulator Zar1l is required to control the timing of meiosis in Xenopus oocytes