PXD032161-1

PXD032161 is an original dataset announced via ProteomeXchange.

Title	Cell type-specific biotin labeling in vivo resolves regional neuronal and astrocyte proteomic differences in mouse brain
Description	Proteomic profiling of brain cell types using isolation-based strategies pose limitations in resolving cellular phenotypes representative of their native state. We generated a novel mouse line for cell type-specific expression of biotin ligase TurboID, leading to in vivo biotinylation of proteins. Using adenoviral and transgenic approaches to label neurons, we show striking protein biotinylation in neuronal soma and axons throughout the brain and quantified over 2,000 neuron-derived proteins spanning synaptic proteins, transporters, ion channels and disease-relevant druggable targets. Next, we compared Camk2a-neuron and Aldh1l1-astrocyte proteomes and resolved brain region-specific proteomic differences within both cell types, some of which may underlie selective vulnerability to neurological diseases. Leveraging the cellular specificity of proteomic labeling, we used an antibody-based approach to uncover differences in neuron and astrocyte-derived signaling phospho-proteins and cytokines. This approach will facilitate the characterization of cell-type specific proteomes in a diverse number of tissues under both physiological and pathological states.
HostingRepository	PRIDE
AnnounceDate	2022-04-29
AnnouncementXML	Submission_2022-05-04_03:44:07.662.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Duc Duong
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-09 02:27:00	ID requested
⏵ 1	2022-05-04 03:44:08	announced

Dataset with its publication pending

submitter keyword: Mouse, LC-MS/MS, Brain, Proteomics

Srikant Rangaraju
contact affiliation	Srikant Rangaraju, MD MS Associate Professor Department of Neurology Vascular Neurology Division Emory University
contact email	srikant.rangaraju@emory.edu
lab head
Duc Duong
contact affiliation	Center of Neurodegenerative Diseases
contact email	dduong@emory.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD032161

PRIDE project URI

• PRIDE
  1. PXD032161
     1. Label: PRIDE project
     2. Name: Cell type-specific biotin labeling in vivo resolves regional neuronal and astrocyte proteomic differences in mouse brain