PXD032157 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A male-specific steroid hormone controls female reproductive biology in the malaria mosquito |
Description | Insects, unlike vertebrates, are generally believed to lack steroid hormones with functions predominantly associated with adult male biology. In the malaria mosquito Anopheles gambiae, the ecdysteroid 20-hydroxyecdysone (20E) appears to both control egg development in females and induce mating refractoriness and oviposition when sexually transferred by males. Here we show that these sex-specific functions are instead carried out by distinct steroids. We identify a male-specific oxidized form of 20E (3D20E) that upon sexual transfer switches off female mating receptivity, ensuring male paternity. Endogenous female 20E does not induce mating refractoriness, while it triggers oviposition in mated females when expression of a 20E-inhibiting kinase is repressed. 3D20E and 20E have different downstream targets, with 3D20E inducing expression of a tolerance factor that preserves female fitness during Plasmodium infection. The evolution of this male steroid has therefore not only shaped the mating biology of An. gambiae, but also impacted malaria transmission. |
HostingRepository | PRIDE |
AnnounceDate | 2022-04-21 |
AnnouncementXML | Submission_2022-04-21_01:30:04.700.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Gennifer Merrihew |
SpeciesList | scientific name: Anopheles gambiae str. PEST; NCBI TaxID: 180454; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-03-09 02:23:47 | ID requested | |
⏵ 1 | 2022-04-21 01:30:05 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: LC-MS/MS, Mosquito, Ejaculome, QE-HF, malaria |
Contact List
Michael MacCoss |
contact affiliation | MacCoss Lab University of Washington Department of Genome Sciences Seattle, WA USA |
contact email | maccoss@uw.edu |
lab head | |
Gennifer Merrihew |
contact affiliation | Dept of Genome Sciences, University of Washington |
contact email | genn@uw.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD032157
- Label: PRIDE project
- Name: A male-specific steroid hormone controls female reproductive biology in the malaria mosquito