PXD032144-1

PXD032144 is an original dataset announced via ProteomeXchange.

Title	EXTL3ΔN purification from EXT1 CRISPR HEK
Description	Experiment for label-free quantification of contaminants in preparations of EXTL3ΔN from transgenic HEK cells. EXTL3ΔN-expressing HEK cells were not untreated (untransfected / Batch C), treated with a set of CRISPR control plasmids (CRISPR control / Batch A), or treated with a set of CRISPR plasmids targeting EXT1 (CRISPR EXT1 / Batch B). EXTL3ΔN was then purified by HisTrap and size exclusion before analysing by mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2022-07-12
AnnouncementXML	Submission_2022-07-12_07:31:16.438.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD032144
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Louis Wilson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-09 02:05:39	ID requested
⏵ 1	2022-07-12 07:31:17	announced

Wilson LFL, Dendooven T, Hardwick SW, Echevarr, í, a-Poza A, Tryfona T, Krogh KBRM, Chirgadze DY, Luisi BF, Logan DT, Mani K, Dupree P, The structure of EXTL3 helps to explain the different roles of bi-domain exostosins in heparan sulfate synthesis. Nat Commun, 13(1):3314(2022) [pubmed]

submitter keyword: HEK, EXTL3, EXTL3ΔN, EXT1

Paul Dupree
contact affiliation	Department of Biochemistry, University of Cambridge, United Kingdom
contact email	pd101@cam.ac.uk
lab head
Louis Wilson
contact affiliation	University of Virginia
contact email	man6ny@virginia.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD032144

PRIDE project URI

• PRIDE
  1. PXD032144
     1. Label: PRIDE project
     2. Name: EXTL3ΔN purification from EXT1 CRISPR HEK