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PXD032144 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEXTL3ΔN purification from EXT1 CRISPR HEK
DescriptionExperiment for label-free quantification of contaminants in preparations of EXTL3ΔN from transgenic HEK cells. EXTL3ΔN-expressing HEK cells were not untreated (untransfected / Batch C), treated with a set of CRISPR control plasmids (CRISPR control / Batch A), or treated with a set of CRISPR plasmids targeting EXT1 (CRISPR EXT1 / Batch B). EXTL3ΔN was then purified by HisTrap and size exclusion before analysing by mass spectrometry.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterLouis Wilson
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-03-09 02:05:39ID requested
12022-07-12 07:31:17announced
Publication List
Wilson LFL, Dendooven T, Hardwick SW, Echevarr, í, a-Poza A, Tryfona T, Krogh KBRM, Chirgadze DY, Luisi BF, Logan DT, Mani K, Dupree P, The structure of EXTL3 helps to explain the different roles of bi-domain exostosins in heparan sulfate synthesis. Nat Commun, 13(1):3314(2022) [pubmed]
Keyword List
submitter keyword: HEK, EXTL3, EXTL3ΔN, EXT1
Contact List
Paul Dupree
contact affiliationDepartment of Biochemistry, University of Cambridge, United Kingdom
contact emailpd101@cam.ac.uk
lab head
Louis Wilson
contact affiliationUniversity of Virginia
contact emailman6ny@virginia.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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