PXD032085 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Global post-translational modification profiling of HIV-1-infected cells reveals mechanisms of host cellular pathway remodeling |
Description | Viruses must effectively remodel host cellular pathways to replicate and evade immune defenses, and they must do so with limited genomic coding capacity. Targeting post-translational modification (PTM) pathways provides a mechanism by which viruses can broadly and rapidly transform a hostile host environment into a hospitable one. We used mass spectrometry-based proteomics to quantify changes in protein abundance and two PTM types – phosphorylation and ubiquitination – in response to HIV-1 infection with viruses harboring targeted deletions of a subset of HIV-1 genes. PTM analysis revealed a requirement for Aurora kinase activity in HIV-1 infection and identified substrates of a phosphatase that is degraded during infection. Finally, we demonstrated that the Cullin4A-DDB1-DCAF1 E3 ubiquitin ligase ubiquitinates histone H1 somatic isoforms and that the HIV-1 Vpr protein inhibits this process, leading to defects in DNA repair. |
HostingRepository | PRIDE |
AnnounceDate | 2023-07-13 |
AnnouncementXML | Submission_2023-07-13_01:30:46.389.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | JeffreyJohnson |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-03-05 12:16:10 | ID requested | |
1 | 2022-04-14 05:58:51 | announced | |
⏵ 2 | 2023-07-13 01:30:47 | announced | 2023-07-13: Updated project metadata. |
Publication List
Johnson JR, Crosby DC, Hultquist JF, Kurland AP, Adhikary P, Li D, Marlett J, Swann J, H, ü, ttenhain R, Verschueren E, Johnson TL, Newton BW, Shales M, Simon VA, Beltrao P, Frankel AD, Marson A, Cox JS, Fregoso OI, Young JAT, Krogan NJ, Global post-translational modification profiling of HIV-1-infected cells reveals mechanisms of host cellular pathway remodeling. Cell Rep, 39(2):110690(2022) [pubmed] |
Keyword List
submitter keyword: HIV-1, proteomics, vif, b56, ubiquitination, vpr, pp2a, phosphorylation, histone h1, systems biology |
Contact List
JeffreyJohnson |
contact affiliation | Department of Microbiology; Icahn School of Medicine at Mount Sinai; New York, NY, 10029; USA |
contact email | Jeffrey.Johnson@mssm.edu |
lab head | |
JeffreyJohnson |
contact affiliation | Icahn School of Medicine at Mount Sinai |
contact email | jeffrey.johnson@mssm.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD032085
- Label: PRIDE project
- Name: Global post-translational modification profiling of HIV-1-infected cells reveals mechanisms of host cellular pathway remodeling