PXD032084-1

PXD032084 is an original dataset announced via ProteomeXchange.

Title	Small Intestinal Resident Eosinophils Maintain Gut Homeostasis Following Microbial Colonisation
Description	Intestinal homeostasis following postnatal microbial colonization requires the coordination of multiple processes, including the activation of immune cells, cell-cell communication, the controlled deposition of extracellular matrix, and epithelial cell turnover and differentiation. The intestine harbors the largest frequency of resident eosinophils of all homeostatic organs, yet the functional significance of eosinophil residence in the gut remains unclear. Eosinophils are equipped to both respond to, and modify, their local tissue environment and thus are able to regulate the adaption of tissues to environmental changes. We report a critical role for eosinophils in regulating villous structure, barrier integrity and motility in the small intestine. Notably, the microbiota was identified as a key driver of small intestinal eosinophil activation and function. Collectively our findings demonstrate a critical role for eosinophils in facilitating mutualistic interactions between host and microbiota and provide a rationale for the functional significance of their early life recruitment in the small intestine.
HostingRepository	PRIDE
AnnounceDate	2025-08-27
AnnouncementXML	Submission_2025-08-27_08:02:16.091.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Antoine Dufour
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-05 12:15:41	ID requested
⏵ 1	2025-08-27 08:02:17	announced

Ignacio A, Shah K, Bernier-Latmani J, K, ö, ller Y, Coakley G, Moyat M, Hamelin R, Armand F, Wong NC, Ramay H, Thomson CA, Burkhard R, Wang H, Dufour A, Geuking MB, McDonald B, Petrova TV, Harris NL, McCoy KD, Small intestinal resident eosinophils maintain gut homeostasis following microbial colonization. Immunity, 55(7):1250-1267.e12(2022) [pubmed]

10.1016/j.immuni.2022.05.014;

submitter keyword: • Eosinophil • Tissue homeostasis • Intestinal barrier • Small intestine • Germ-free • Microbiome • Extracellular matrix • Villous atrophy

Antoine Dufour and Kathy McCoy
contact affiliation	Physiology and Pharmacology, University of Calgary
contact email	antoine.dufour@ucalgary.ca
lab head
Antoine Dufour
contact affiliation	University of Calgary Department of Physiology and Pharmacology
contact email	antoine.dufour@ucalgary.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD032084

PRIDE project URI

• PRIDE
  1. PXD032084
     1. Label: PRIDE project
     2. Name: Small Intestinal Resident Eosinophils Maintain Gut Homeostasis Following Microbial Colonisation