⮝ Full datasets listing

PXD032083-1

PXD032083 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of arginine phosphorylation in Mycolicibacterium smegmatis by shotgun proteomics
Descriptionuberculosis is a leading cause of worldwide infectious mortality. The prevalence of multidrug-resistant Mycobacterium tuberculosis (Mtb) infections drives an urgent need to exploit new drug targets. One such target is the ATP-dependent protease ClpC1P1P2, which is strictly essential for viability. However, few proteolytic substrates of mycobacterial ClpC1P1P2 have been identified to date. Recent studies in Bacillus subtilis have shown that the orthologous ClpCP protease recognizes proteolytic substrates bearing post-translational arginine phosphorylation. While several lines of evidence suggest that ClpC1P1P2 is similarly capable of recognizing phosphoarginine-bearing proteins, the existence of phosphoarginine modifications in mycobacteria has remained in question. Here, we confirm the presence of post-translational phosphoarginine modifications in Mycolicibacterium smegmatis (Msm), a nonpathogenic surrogate of Mtb. Using a phosphopeptide enrichment workflow coupled with shotgun phosphoproteomics, we identify arginine phosphosites on specific targets within the Msm proteome. In all, our findings provide new evidence supporting the existence of phosphoarginine modification in mycobacteria and other actinobacterial species.
HostingRepositoryPRIDE
AnnounceDate2022-09-30
AnnouncementXMLSubmission_2022-09-30_10:53:15.472.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD032083
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterEmmanuel Ogbonna
SpeciesList scientific name: Mycolicibacterium smegmatis MC2 155; NCBI TaxID: 246196;
ModificationListphosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-03-05 12:15:13ID requested
12022-09-30 10:53:15announced
22023-11-14 08:55:22announced2023-11-14: Updated project metadata.
Publication List
10.1128/SPECTRUM.02042-22;
Keyword List
submitter keyword: Mycobacterium tuberculosis, Mycolicibacterium smegmatis, arginine phosphorylation, posttranslation modification, shotgun proteomics, LC-MS/MS
Contact List
Karl Schmitz
contact affiliationDepartment of Biological Sciences Department of Chemistry & Biochemistry University of Delaware Newark DE, 19716
contact emailschmitzk@udel.edu
lab head
Emmanuel Ogbonna
contact affiliationUniversity of Delaware
contact emaileogbonna@udel.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD032083
PRIDE project URI
Repository Record List
[ + ]