PXD032083-1

PXD032083 is an original dataset announced via ProteomeXchange.

Title	Identification of arginine phosphorylation in Mycolicibacterium smegmatis by shotgun proteomics
Description	uberculosis is a leading cause of worldwide infectious mortality. The prevalence of multidrug-resistant Mycobacterium tuberculosis (Mtb) infections drives an urgent need to exploit new drug targets. One such target is the ATP-dependent protease ClpC1P1P2, which is strictly essential for viability. However, few proteolytic substrates of mycobacterial ClpC1P1P2 have been identified to date. Recent studies in Bacillus subtilis have shown that the orthologous ClpCP protease recognizes proteolytic substrates bearing post-translational arginine phosphorylation. While several lines of evidence suggest that ClpC1P1P2 is similarly capable of recognizing phosphoarginine-bearing proteins, the existence of phosphoarginine modifications in mycobacteria has remained in question. Here, we confirm the presence of post-translational phosphoarginine modifications in Mycolicibacterium smegmatis (Msm), a nonpathogenic surrogate of Mtb. Using a phosphopeptide enrichment workflow coupled with shotgun phosphoproteomics, we identify arginine phosphosites on specific targets within the Msm proteome. In all, our findings provide new evidence supporting the existence of phosphoarginine modification in mycobacteria and other actinobacterial species.
HostingRepository	PRIDE
AnnounceDate	2022-09-30
AnnouncementXML	Submission_2022-09-30_10:53:15.472.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD032083
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Emmanuel Ogbonna
SpeciesList	scientific name: Mycolicibacterium smegmatis MC2 155; NCBI TaxID: 246196;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-05 12:15:13	ID requested
⏵ 1	2022-09-30 10:53:15	announced
2	2023-11-14 08:55:22	announced	2023-11-14: Updated project metadata.

10.1128/SPECTRUM.02042-22;

submitter keyword: Mycobacterium tuberculosis, Mycolicibacterium smegmatis, arginine phosphorylation, posttranslation modification, shotgun proteomics, LC-MS/MS

Karl Schmitz
contact affiliation	Department of Biological Sciences Department of Chemistry & Biochemistry University of Delaware Newark DE, 19716
contact email	schmitzk@udel.edu
lab head
Emmanuel Ogbonna
contact affiliation	University of Delaware
contact email	eogbonna@udel.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032083
     1. Label: PRIDE project
     2. Name: Identification of arginine phosphorylation in Mycolicibacterium smegmatis by shotgun proteomics