PXD032081-1

PXD032081 is an original dataset announced via ProteomeXchange.

Title	Peptide of Trichinella spiralis infective larval extract that harnesses growth of human hepatoma cells
Description	This project aimed to explore novel anticancer therapeutics from products of parasite since several data related to benefits from the T. spiralis infection have been documented. We validated antitumor activity of T. spiralis infective larval extract and extricate the parasite-derived-antitumor peptide. We found that larval extract exerted antitumor activity to three types of carcinoma cells including hepatocellular carcinoma HepG2, ovarian cancer SK-OV-3, and lung adenocarcinoma A549. Interestingly, it displayed the most antitumor effect to HepG2 cells. Using proteomic and bioinformatic approaches, three putative anticancer peptides were identified from T. spiralis infective larval extract. One of these peptides showed a dose-dependent-anti-HepG2 effect by inducing ROS accumulation, leading to inhibition of the cell proliferation. Our data indicate potential application of the larval extract-derived antitumor peptide as a complementary agent for human hepatoma treatment and highlight a positive aspect of parasite apart from its deleterious effect.
HostingRepository	PRIDE
AnnounceDate	2022-04-13
AnnouncementXML	Submission_2022-04-13_03:36:51.642.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pichet Ruenchit
SpeciesList	scientific name: Trichinella spiralis; NCBI TaxID: 6334;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-05 12:14:23	ID requested
⏵ 1	2022-04-13 03:36:52	announced

Dataset with its publication pending

submitter keyword: Antitumor peptide, Drug discovery, Human hepatocellular carcinoma HepG2 cell, Infective larva, Proteomics, Trichinella spiralis

Pichet Ruenchit
contact affiliation	Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
contact email	pichet.rue@mahidol.edu
lab head
Pichet Ruenchit
contact affiliation	Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
contact email	pichet.rue@mahidol.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD032081

PRIDE project URI

• PRIDE
  1. PXD032081
     1. Label: PRIDE project
     2. Name: Peptide of Trichinella spiralis infective larval extract that harnesses growth of human hepatoma cells