PXD032072-1

PXD032072 is an original dataset announced via ProteomeXchange.

Title	The PripA-TbcrA complex-centered Rab GAP cascade facilitates macropinosome maturation in Dictyostelium
Description	Macropinocytosis, an evolutionarily conserved mechanism mediating nonspecific bulk uptake of extracellular fluid, has been ascribed diverse functions. How nascent macropinosomes mature after internalization remains largely unknown. By searching for proteins that localize on macropinosomes during the Rab5-to-Rab7 transition stage in Dictyostelium, we uncover a complex composed of two proteins, which we name PripA and TbcrA. We show that the Rab5-to-Rab7 conversion involves fusion of Rab5-marked early macropinosomes with Rab7-marked late macropinosomes. PripA links the two membrane compartments by interacting with PI(3,4)P2 and Rab7. In addition, PripA recruits TbcrA, which acts as a GAP, to turn off Rab5. Thus, the conversion to Rab7 is linked to inactivation of the upstream Rab5. Consistently, disruption of either pripA or tbcrA impairs Rab5 inactivation and macropinocytic cargo processing. Therefore, the PripA-TbcrA complex is the central component of a Rab GAP cascade that facilitates programmed Rab switch and efficient cargo trafficking during macropinosome maturation.
HostingRepository	PRIDE
AnnounceDate	2022-03-14
AnnouncementXML	Submission_2022-03-14_02:41:42.541.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hui Tu
SpeciesList	scientific name: Dictyostelium discoideum (Slime mold); NCBI TaxID: 44689;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-05 05:20:26	ID requested
⏵ 1	2022-03-14 02:41:42	announced

Dataset with its publication pending

submitter keyword: macropinocytosis, PripA, TbcrA, Rab5, Rab7

Huaqing Cai
contact affiliation	Institute of Biophysics, Chinese Academy of Sciences
contact email	huaqingcai@ibp.ac.cn
lab head
Hui Tu
contact affiliation	Insititute of Biophysics
contact email	18070285210@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032072
     1. Label: PRIDE project
     2. Name: The PripA-TbcrA complex-centered Rab GAP cascade facilitates macropinosome maturation in Dictyostelium