PXD032017-3

PXD032017 is an original dataset announced via ProteomeXchange.

Title	An Amphipathic Helix in Brl1 is Required for Nuclear Pore Complex Biogenesis in S. cerevisiae - KARMA analysis of Brl1 (DIA)
Description	This project was aimed at analyzing the binding of Brl1 to the nuclear pore complex during the assembly process using the Kinetic Analysis of incorporation Rates in Macromolecular Assemblies (KARMA, Onischenko et al. 2020) method. In brief, we affinity purified Brl1 at several time points following SILAC metabolic labeling in growing S. cerevisiae cultures. Then, we analyzed the metabolic labeling for all co-purified nucleoporins and used these labeling kinetics to infer the timing of Brl1 binding during the native nuclear pore complex assembly.
HostingRepository	PRIDE
AnnounceDate	2025-01-07
AnnouncementXML	Submission_2025-01-06_16:39:22.676.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Federico Uliana
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-02 10:02:28	ID requested
1	2022-08-17 06:43:44	announced
2	2024-10-22 05:39:12	announced	2024-10-22: Updated project metadata.
⏵ 3	2025-01-06 16:39:23	announced	2025-01-07: Updated project metadata.

10.7554/elife.78385;

Kralt A, Wojtynek M, Fischer JS, Agote-Aran A, Mancini R, Dultz E, Noor E, Uliana F, Tatarek-Nossol M, Antonin W, Onischenko E, Medalia O, Weis K, . Elife, 11():(2022) [pubmed]

submitter keyword: DIA,KARMA

Federico Uliana
contact affiliation	Institute of Biochemistry (IBC, ETHZ)
contact email	federico.uliana@bc.biol.ethz.ch
lab head
Federico Uliana
contact affiliation	ETH
contact email	uliana@imsb.biol.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD032017

PRIDE project URI

• PRIDE
  1. PXD032017
     1. Label: PRIDE project
     2. Name: An Amphipathic Helix in Brl1 is Required for Nuclear Pore Complex Biogenesis in S. cerevisiae - KARMA analysis of Brl1 (DIA)