PXD032016-2

PXD032016 is an original dataset announced via ProteomeXchange.

Title	An Amphipathic Helix in Brl1 is Required for Nuclear Pore Complex Biogenesis in S. cerevisiae – targeted analysis of cell lysate (PRM)
Description	The project was aimed at assessing the metabolic labeling of NUPs in the full lysate upon SILAC labeling of growing yeast cultures, 90 min post labeling. This experiment was performed to exclude that the systematic labeling differences that we observed in KARMA assays with Brl1 bait were present already in the source lysate.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:39:13.128.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Federico Uliana
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-02 09:55:14	ID requested
1	2022-08-17 06:35:17	announced
⏵ 2	2024-10-22 05:39:13	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: cell lysate,PRM

Federico Uliana
contact affiliation	Institute of Biochemistry (IBC, ETHZ)
contact email	federico.uliana@bc.biol.ethz.ch
lab head
Federico Uliana
contact affiliation	ETH
contact email	uliana@imsb.biol.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD032016

PRIDE project URI

• PRIDE
  1. PXD032016
     1. Label: PRIDE project
     2. Name: An Amphipathic Helix in Brl1 is Required for Nuclear Pore Complex Biogenesis in S. cerevisiae – targeted analysis of cell lysate (PRM)