PXD032015-2

PXD032015 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry analysis of the impact of replication stress upon phosphorylation and protein interaction of human RIF1 protein
Description	Wild-type or S2265A version of GFP-RIF1 protein (isoform 1) was over-expressed in Flp-In-T-REx cells (Watts et al. 2020. eLife 9:e58020) and immunopurified using GFP-Trap magnetic agarose beads. Proteins were subjected to on-beads trypsin digestion and resulting peptides were analysed by mass spectrometry for protein identification/quantification and identification of phosphorylated residues. Raw MS file names and descriptions: IP_RIF1_A.raw = GFP-RIF1-L immunoprecipitated from cells without aphidicolin treatment. IP_RIF1_B.raw = GFP-RIF1-L immunoprecipitated from cells treated with 1 µM aphidicolin for 24 hr before harvesting. IP_RIF1_C.raw = GFP-RIF1-L (S2265A) immunoprecipitated from cells treated with 1 µM aphidicolin for 24 hr before harvesting.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:55:50.119.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Stead
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-02 09:53:12	ID requested
1	2022-04-14 07:52:33	announced
⏵ 2	2023-11-14 08:55:51	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: RIF1, protein phosphorylation, replication stress

Dr Shin-ichiro Hiraga
contact affiliation	Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, United Kingdom
contact email	s.hiraga@abdn.ac.uk
lab head
David Stead
contact affiliation	University of Aberdeen
contact email	d.stead@abdn.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD032015

PRIDE project URI

• PRIDE
  1. PXD032015
     1. Label: PRIDE project
     2. Name: Mass spectrometry analysis of the impact of replication stress upon phosphorylation and protein interaction of human RIF1 protein