PXD032012-1

PXD032012 is an original dataset announced via ProteomeXchange.

Title	Malaria-infected cells release distinct vesicle subsets featuring markers of different destinations
Description	Abstract Malaria is the most serious mosquito-borne parasitic disease, caused mainly by the intracellular parasite Plasmodium falciparum. This parasite invades human red blood cells and releases extracellular vesicles (EVs) to alter its host responses. It becomes clear that EVs are generally composed of sub-populations. Seeking to identify the various EV subpopulations we subjected malaria-derived EVs to size-separation analysis, using Asymmetric Flow Field-Flow Fractionation. Multi-technique analysis revealed surprising characteristics: two distinct EV subpopulations differing in size and protein content were identified. The small EVs were enriched in complement-system proteins and the large EVs with proteasome subunits. We then measured the membrane fusion abilities of each subpopulation with three types of host cellular membranes: plasma, late and early endosome. Remarkably, small EVs fused to early-endosome liposomes at significantly greater levels than large EVs. Combined atomic force microscope with machine-learning methods further emphasize the difference in biophysical properties between the two subpopulations. These results shed light on the sophisticated mechanism by which malaria parasites utilize EV subpopulations as a communication tool to target different cellular destinations or host systems.
HostingRepository	PRIDE
AnnounceDate	2022-07-01
AnnouncementXML	Submission_2022-07-01_05:08:54.957.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tamar Ziv
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-02 09:46:53	ID requested
⏵ 1	2022-07-01 05:08:55	announced

Dataset with its publication pending

submitter keyword: Malaria, vesicle

Neta Regev-Rudzki
contact affiliation	Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot 7610001, Israel
contact email	neta.regev-rudzki@weizmann.ac.il
lab head
Tamar Ziv
contact affiliation	Technion
contact email	tamarz@technion.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD032012

PRIDE project URI

• PRIDE
  1. PXD032012
     1. Label: PRIDE project
     2. Name: Malaria-infected cells release distinct vesicle subsets featuring markers of different destinations