PXD031989-1

PXD031989 is an original dataset announced via ProteomeXchange.

Title	Proteomic comparison of Biomphalaria glabrata excretory-secretory proteins at different time-points of Schistosoma mansoni infection
Description	Schistosomiasis, caused by infection with Schistosoma trematode parasites, is one of the deadliest neglected tropical diseases in the world. The Schistosoma lifecycle involves the miracidia infection of an intermediate molluscan host, such as Biomphalaria glabrata. Dispersing B. glabrata attractant chemicals has been considered a method of minimising host infections. The attractiveness of B. glabrata is known to be reduced following infection; however, it remained unclear how the duration of infection affects attractiveness. Identifying excretory-secretory proteins (ESPs) abundant in attractive snail conditioned water (SCW) may facilitate the identification of attractants. This study obtained SCW ESPs from naïve snails and at different time-points post-miracidia exposure (PME; 16h-, 1 week, 2 weeks and 3 weeks), to identify those ESPs mediating Schistosome mansoni miracidia behavioural changes. The ESPs were quantitatively identified from all SCW samples using a label-free proteomic method and genome-derived protein databases of B. glabrata and S. mansoni. We found that changes in miracidia behaviour were similar upon exposure to naïve and 3W-PME SCW, including increased circling and quantity of miracidia in the field of view. Of the ESPs identified, 21 were shared in SCW of naïve and 3W-PME, and considered attractant candidates, including acetylcholine-binding protein and immune-related proteins such as biomphalysins and thioester-containing protein 1. Some biological processes were exclusive to attractant candidates, including obsolete pathogenesis and transmembrane transport activity. This suggests that compromised production of these proteins may diminish host attractiveness to miracidia. Additionally, several immune proteins were identified exclusively at 16-PME and 1W-PME, providing further information into changing B. glabrata immune responses throughout infection. These findings provide a list of attractant candidates to potentially decrease B. glabrata infection and minimise schistosomiasis.
HostingRepository	PRIDE
AnnounceDate	2022-10-03
AnnouncementXML	Submission_2022-10-03_03:53:01.526.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tianfang Wang
SpeciesList	scientific name: Biomphalaria glabrata; NCBI TaxID: 6526; scientific name: Schistosoma mansoni; NCBI TaxID: 6183;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	X500R QTOF

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-02 01:13:05	ID requested
⏵ 1	2022-10-03 03:53:01	announced
2	2023-11-14 08:55:19	announced	2023-11-14: Updated project metadata.

10.3389/FIMMU.2022.954282;

submitter keyword: Biomphalaria glabrata, excretory-secretory proteins, Schistosoma mansoni, LC-MS/MS

Tianfang Wang
contact affiliation	Centre for Bioinnovation, University of the Sunshine Coast
contact email	twang@usc.edu.au
lab head
Tianfang Wang
contact affiliation	University of the Sunshine Coast
contact email	twang@usc.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031989
     1. Label: PRIDE project
     2. Name: Proteomic comparison of Biomphalaria glabrata excretory-secretory proteins at different time-points of Schistosoma mansoni infection