PXD031972 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Protection of nascent DNA at stalled replication forks is mediated by phosphorylation of RIF1 intrinsically disordered region |
Description | RIF1 is a multifunctional protein that plays key roles in the regulation of DNA processing. During repair of DNA double-strand breaks (DSBs), RIF1 functions in the 53BP1-Shieldin pathway that inhibits resection of DNA ends to modulate the cellular decision on which repair pathway to engage. Under conditions of replication stress, RIF1 protects nascent DNA at stalled replication forks from degradation by the DNA2 nuclease. How these RIF1 activities are regulated at the post-translational level has not yet been elucidated. Here, we identified a cluster of conserved ATM/ATR consensus SQ motifs within the intrinsically disordered region (IDR) of mouse RIF1 that are phosphorylated in proliferating B lymphocytes. We found that phosphorylation of the conserved IDR SQ cluster is dispensable for the inhibition of DSB resection by RIF1, but is essential to counteract DNA2-dependent degradation of nascent DNA at stalled replication forks. Therefore, our study identifies a key molecular feature that enables the genome-protective function of RIF1 during DNA replication stress. |
HostingRepository | PRIDE |
AnnounceDate | 2022-04-14 |
AnnouncementXML | Submission_2022-04-14_05:39:00.379.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Oliver Popp |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | phosphorylated residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-03-01 10:23:56 | ID requested | |
⏵ 1 | 2022-04-14 05:39:00 | announced | |
2 | 2023-11-14 08:55:39 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
submitter keyword: RIF1, SQ motifs, intrinsically disordered region, DSB resection inhibition, DNA replication fork protection |
Contact List
Michela Di Virgilio |
contact affiliation | Laboratory of Genome Diversification and Integrity, Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin 13125, Germany |
contact email | Michela.DiVirgilio@mdc-berlin.de |
lab head | |
Oliver Popp |
contact affiliation | MDC Berlin |
contact email | oliver.popp@mdc-berlin.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD031972
- Label: PRIDE project
- Name: Protection of nascent DNA at stalled replication forks is mediated by phosphorylation of RIF1 intrinsically disordered region