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PXD031972-1

PXD031972 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProtection of nascent DNA at stalled replication forks is mediated by phosphorylation of RIF1 intrinsically disordered region
DescriptionRIF1 is a multifunctional protein that plays key roles in the regulation of DNA processing. During repair of DNA double-strand breaks (DSBs), RIF1 functions in the 53BP1-Shieldin pathway that inhibits resection of DNA ends to modulate the cellular decision on which repair pathway to engage. Under conditions of replication stress, RIF1 protects nascent DNA at stalled replication forks from degradation by the DNA2 nuclease. How these RIF1 activities are regulated at the post-translational level has not yet been elucidated. Here, we identified a cluster of conserved ATM/ATR consensus SQ motifs within the intrinsically disordered region (IDR) of mouse RIF1 that are phosphorylated in proliferating B lymphocytes. We found that phosphorylation of the conserved IDR SQ cluster is dispensable for the inhibition of DSB resection by RIF1, but is essential to counteract DNA2-dependent degradation of nascent DNA at stalled replication forks. Therefore, our study identifies a key molecular feature that enables the genome-protective function of RIF1 during DNA replication stress.
HostingRepositoryPRIDE
AnnounceDate2022-04-14
AnnouncementXMLSubmission_2022-04-14_05:39:00.379.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterOliver Popp
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListphosphorylated residue
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-03-01 10:23:56ID requested
12022-04-14 05:39:00announced
22023-11-14 08:55:39announced2023-11-14: Updated project metadata.
Publication List
10.7554/ELIFE.75047;
Keyword List
submitter keyword: RIF1, SQ motifs, intrinsically disordered region, DSB resection inhibition, DNA replication fork protection
Contact List
Michela Di Virgilio
contact affiliationLaboratory of Genome Diversification and Integrity, Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin 13125, Germany
contact emailMichela.DiVirgilio@mdc-berlin.de
lab head
Oliver Popp
contact affiliationMDC Berlin
contact emailoliver.popp@mdc-berlin.de
dataset submitter
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Dataset FTP location
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