PXD031967-1

PXD031967 is an original dataset announced via ProteomeXchange.

Title	Stable Isotope Labelling with Amino Acids in Cell Culture – Size Exclusion Chromatography – Mass Spectrometry
Description	Most proteins execute their functions through interacting with other macromolecules, and protein complexes formed by organized protein-protein interactions represent a primary functional module in the cells. Recently, People combine several proteomic techniques including cell fractionation, protein chromatography, and quantitative mass spectrometry (MS) to analyze the study composition and dynamics of protein complexes. To quantify the change of protein complex by this method, we combine it with the SILAC technique to detect the difference between different isotope-labeling samples. Here we harvest Saccharomyces cerevisiae cells in heavy, medium, and light labeling mediums. Then, extract the protein from cell pellets and separate it into 27 fractions by Size-exclusion chromatography. Using LC-MS/MS to detect protein from each fraction for doing protein complexes analysis
HostingRepository	PRIDE
AnnounceDate	2025-11-17
AnnouncementXML	Submission_2025-11-16_18:16:13.762.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	chien fu liu
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-01 10:14:09	ID requested
⏵ 1	2025-11-16 18:16:20	announced

10.1093/gigascience/giaf122;

Chen YH, Liu CF, Leu JY, Tsai HK, Complete end-to-end learning from protein feature representation to protein interactome inference. Gigascience, 14():(2025) [pubmed]

submitter keyword: yeast, MS,SILAC

Jun Yi Leu
contact affiliation	Institute of Molecular Biology, Academia Sinica, Taiwan
contact email	jleu@imb.sinica.edu.tw
lab head
chien fu liu
contact affiliation	Institute of Molecular Biology, Academia Sinica
contact email	jeff790906@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD031967

PRIDE project URI

• PRIDE
  1. PXD031967
     1. Label: PRIDE project
     2. Name: Stable Isotope Labelling with Amino Acids in Cell Culture – Size Exclusion Chromatography – Mass Spectrometry