PXD031955-1

PXD031955 is an original dataset announced via ProteomeXchange.

Title	Capillary Electrophoresis Mass Spectrometry for Scalable Single-Cell Proteomics
Description	This project used a custom-built capillary electrophoresis (CE) mass spectrometry (MS) platform to demonstrate the scalable analysis of proteins from single cells of varying sizes and protein content in different vertebrate biological models. Neural tissue fated giant cells were identified in cleavage-stage Xenopus laevis (frog) embryos, and 18 ng from its protein content was analyzed. From cultured primary neurons from the mouse, diluted samples containing ~125 pg of protein digest was measured, estimating to a portion of the somal protein content. Compared to traditional nano-flow liquid chromatography (nanoLC) MS, CE-MS provided higher sensitivity and faster analysis. CE-ESI-HRMS offers a viable approach for scalable single-cell proteomics.
HostingRepository	PRIDE
AnnounceDate	2022-03-02
AnnouncementXML	Submission_2022-03-02_10:04:54.709.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Nemes
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-28 12:26:26	ID requested
⏵ 1	2022-03-02 10:04:55	announced

Dataset with its publication pending

submitter keyword: Capillary electrophoresis, scalability, proteomics, subcellular, mass spectrometry

Peter Nemes
contact affiliation	Department of Chemistry & Biochemistry, University of Maryland, College Park, MD 20742, USA
contact email	nemes@umd.edu
lab head
Peter Nemes
contact affiliation	University of Maryland, College Park
contact email	nemes@umd.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD031955

PRIDE project URI

• PRIDE
  1. PXD031955
     1. Label: PRIDE project
     2. Name: Capillary Electrophoresis Mass Spectrometry for Scalable Single-Cell Proteomics