PXD031924-1

PXD031924 is an original dataset announced via ProteomeXchange.

Title	Equine muscle-derived mesenchymal stem cells protect cartilage explants in osteoarthritis model: secretome characterization
Description	Background: Osteoarthritis is a highly prevalent joint degenerative disease for which therapeutic treatments are limited or invasive. Cell therapy based on mesenchymal stem/stromal cells (MSCs) is therefore seen as a promising approach for this disease, in both human and horses. Objective: As the regenerative potential of MSCs is mainly conferred by paracrine function, the goal of this study is to characterize the secretome of muscle-derived MSCs (mdMSCs) in an in vitro model of OA to evaluate the clinical interest of mdMSCs as cell therapy for joint diseases like osteoarthritis. Methods: An equine osteoarthritis model composed of cartilage explants exposed to pro-inflammatory cytokines was first developed. Then, the effects of mdMSC coculture on cartilage explant were studied by measuring the glycosaminoglycan release and the NO2- production. To identify the underlying molecular actors, SILAC-based secretome analyses were conducted, in the presence of serum. The relative abundance of highly sequenced proteins was finally confirmed by western blot. Results: Co-culture with muscle-derived MSCs decreases the cytokine-induced glycosaminoglycan release by cartilage explants, suggesting a protecting effect of mdMSCs. Among the 52 equine proteins sequenced in the co-culture medium, the abundance of decorin and matrix metalloproteinase 3 was modified, as confirmed by western blot analyses. Conclusions: These results suggest that muscle-derived MSCs could reduce the catabolic effect of TNF and IL-1 on cartilage explant by decreasing the secretion and activity of Matrix Metalloproteinase 3 and increasing the decorin secretion
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_13:45:26.757.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD031924
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Marc Dieu
SpeciesList	scientific name: Equus caballus (Horse); NCBI TaxID: 9796;
ModificationList	(4,4,5,5-(2)H4)-L-lysine; 6x(13)C labeled residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-28 02:52:34	ID requested
⏵ 1	2024-10-07 13:45:27	announced

10.6019/PXD031924;

10.1007/S12015-022-10463-4;

submitter keyword: stem cells

equine, secretome

Patricia RENARD
contact affiliation	Université de Namur - URBC Narilis
contact email	patsy.renard@unamur.be
lab head
Marc Dieu
contact affiliation	University of Namur - MaSUN
contact email	marc.dieu@unamur.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031924
     1. Label: PRIDE project
     2. Name: Equine muscle-derived mesenchymal stem cells protect cartilage explants in osteoarthritis model: secretome characterization