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PXD031924-1

PXD031924 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEquine muscle-derived mesenchymal stem cells protect cartilage explants in osteoarthritis model: secretome characterization
DescriptionBackground: Osteoarthritis is a highly prevalent joint degenerative disease for which therapeutic treatments are limited or invasive. Cell therapy based on mesenchymal stem/stromal cells (MSCs) is therefore seen as a promising approach for this disease, in both human and horses. Objective: As the regenerative potential of MSCs is mainly conferred by paracrine function, the goal of this study is to characterize the secretome of muscle-derived MSCs (mdMSCs) in an in vitro model of OA to evaluate the clinical interest of mdMSCs as cell therapy for joint diseases like osteoarthritis. Methods: An equine osteoarthritis model composed of cartilage explants exposed to pro-inflammatory cytokines was first developed. Then, the effects of mdMSC coculture on cartilage explant were studied by measuring the glycosaminoglycan release and the NO2- production. To identify the underlying molecular actors, SILAC-based secretome analyses were conducted, in the presence of serum. The relative abundance of highly sequenced proteins was finally confirmed by western blot. Results: Co-culture with muscle-derived MSCs decreases the cytokine-induced glycosaminoglycan release by cartilage explants, suggesting a protecting effect of mdMSCs. Among the 52 equine proteins sequenced in the co-culture medium, the abundance of decorin and matrix metalloproteinase 3 was modified, as confirmed by western blot analyses. Conclusions: These results suggest that muscle-derived MSCs could reduce the catabolic effect of TNF and IL-1 on cartilage explant by decreasing the secretion and activity of Matrix Metalloproteinase 3 and increasing the decorin secretion
HostingRepositoryPRIDE
AnnounceDate2024-10-07
AnnouncementXMLSubmission_2024-10-07_13:45:26.757.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD031924
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMarc Dieu
SpeciesList scientific name: Equus caballus (Horse); NCBI TaxID: 9796;
ModificationList(4,4,5,5-(2)H4)-L-lysine; 6x(13)C labeled residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentBruker Daltonics timsTOF series
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-02-28 02:52:34ID requested
12024-10-07 13:45:27announced
Publication List
10.6019/PXD031924;
10.1007/S12015-022-10463-4;
Keyword List
submitter keyword: stem cells
equine, secretome
Contact List
Patricia RENARD
contact affiliationUniversité de Namur - URBC Narilis
contact emailpatsy.renard@unamur.be
lab head
Marc Dieu
contact affiliationUniversity of Namur - MaSUN
contact emailmarc.dieu@unamur.be
dataset submitter
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