PXD031909-1

PXD031909 is an original dataset announced via ProteomeXchange.

Title	Evaluation of transgenic proteins in transformed Camelina sativa leaves
Description	The goal of this study was to detect and quantify the presence of transgenic proteins in transformed C. sativa plants. Experimental plants were transformed with recombinant proteins of a synthetic carbon fixation cycle. Plants expressing the full suite of genes of the synthetic cycle are labeled as “T”. Plants expressing only half of the synthetic cycle are labeled as “C”. Plants transformed with an “empty vector” are labeled as “EV” and serve as a negative control. Five biological replicates were included for each treatment. Additionally, one chloroplast enrichment sample was generated for each line using a commercially available chloroplast isolation kit (Sigma Product #: CPISO). Leaf tissue from photosynthetically-active leaves was harvested and immediately flash frozen with liquid nitrogen. This tissue was stored at -80ºC until it was ground to a fine powder under liquid nitrogen. The tissue was then weighed into 200 mg aliquots. Amino acid sequences of the transgenic proteins were used as a reference and “empty vector” control plants were used as a negative control.
HostingRepository	PRIDE
AnnounceDate	2025-06-30
AnnouncementXML	Submission_2025-06-29_16:11:51.503.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Simina Vintila
SpeciesList	scientific name: Camelina sativa (False flax) (Myagrum sativum); NCBI TaxID: 90675;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-28 01:05:46	ID requested
⏵ 1	2025-06-29 16:11:52	announced

10.3389/fpls.2025.1556957;

Wilson N, Smith-Moore C, Xu Y, Edwards B, La Hovary C, Li K, Aslett D, Ji M, Lin X, Vintila S, Kleiner M, Xie D, Shachar-Hill Y, Grunden A, Sederoff H, characterization of a condensed, reverse TCA (crTCA) cycle. Front Plant Sci, 16():1556957(2025) [pubmed]

submitter keyword: 1D-LC, metaproteome, transgenic proteins, transformed plant, condensed rTCA cycle, metaproteomics

Manuel Kleiner
contact affiliation	North Carolina State University
contact email	manuel_kleiner@ncsu.edu
lab head
Simina Vintila
contact affiliation	North Carolina State University
contact email	svintil@ncsu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD031909

PRIDE project URI

• PRIDE
  1. PXD031909
     1. Label: PRIDE project
     2. Name: Evaluation of transgenic proteins in transformed Camelina sativa leaves