PXD031898-1

PXD031898 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Ammonium Sulfate-based Prefractionation Improved Proteome Coverage in Arabidopsis thaliana Leaf Extract
Description	The hypothesis that ammonium sulfate-based protein precipitation can help prefractionate the total protein extract into fractions of reduced complexity and improve the detection of low-abundance proteins was investigated. This dataset resulted from the experiments that we performed, of which the results validated this hypothesis. Of note, this prefractionation method allowed to identify 45% more proteins compared to the number of proteins identified from the total crude extract without prefractionation. Our results thus showed that a simple offline prefractionation of the proteome by ammonium sulfate prior to liquid chromatography-tandem mass spectrometry drastically improved the detection of intact and post-translationally modified proteins.
HostingRepository	MassIVE
AnnounceDate	2023-02-19
AnnouncementXML	Submission_2023-02-19_10:42:08.429.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Tagnon Missihoun
SpeciesList	scientific name: Arabidopsis thaliana; common name: thale cress; NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-25 11:58:06	ID requested
⏵ 1	2023-02-19 10:42:08	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Arabidopsis thaliana, Ammonium sulafte-based prefractionation, Plant leaves, Orbitrap Fusion, Proteome offline prefractionation

submitter keyword: Arabidopsis thaliana, Ammonium sulafte-based prefractionation, Plant leaves, Orbitrap Fusion, Proteome offline prefractionation

Contact List

Tagnon Missihoun
contact affiliation	University of Quebec Trois-Rivieres
contact email	Tagnon.Missihoun@uqtr.ca
lab head
Tagnon Missihoun
contact affiliation	University of Quebec Trois-Rivieres
contact email	Tagnon.Missihoun@uqtr.ca
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000088920/