PXD031870-1

PXD031870 is an original dataset announced via ProteomeXchange.

Title	BioID proximity labelling of MAP4K4 interactors in DAOY medulloblastoma cells
Description	Mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4) is Ser/Thr protein kinase highly expressed in medulloblastoma (MB) patient samples which promotes cell migration and invasiveness downstream of growth factor receptors. Although MAP4K4 implication in the control of the membrane protrusions at the leading edge of cells during migration and invasion in MB is established, its downstream effectors governing and mediating this process in MB have not been identified. In this study we explored the proteomic neighborhood of MAP4K4 in MB cells and to identify candidate potentially affecting its activity and pro-migratory functions. Towards this aim, we expressed BioID fused versions of MAP4K4 (N- and C-terminal) in DAOY cells under presence of biotin. In the control setup, we expressed the BioID enzyme alone. After Streptavidin purification of biotinylated proteins, we performed mass spectrometry and quantified relative abundances compared to control conditions.
HostingRepository	PRIDE
AnnounceDate	2022-08-04
AnnouncementXML	Submission_2022-08-04_02:07:17.249.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD031870
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Martin Baumgartner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; biotinylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-24 10:02:15	ID requested
⏵ 1	2022-08-04 02:07:17	announced

Dataset with its publication pending

submitter keyword: BioID, MAP4K4, medulloblastoma

Martin Baumgartner
contact affiliation	Pediatric Molecular Neuro-Oncology Research, Department of Oncology, University Children's Hospital Zurich
contact email	Martin.Baumgartner@kispi.uzh.ch
lab head
Martin Baumgartner
contact affiliation	University Children's Hospital Zürich
contact email	Martin.Baumgartner@kispi.uzh.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD031870

PRIDE project URI

• PRIDE
  1. PXD031870
     1. Label: PRIDE project
     2. Name: BioID proximity labelling of MAP4K4 interactors in DAOY medulloblastoma cells