PXD031862-1

PXD031862 is an original dataset announced via ProteomeXchange.

Title	Extracellular vesicles derived from the choroid plexus trigger neural stem cells differentiation
Description	An organized network of beating cilia transports cerebrospinal fluid (CSF) through the ventricles of the brain. CSF takes along diverse solutes including extracellular vesicles (EV), which arise from the choroid plexus, a secretory epithelium that reaches into the ventricles. Along their path through the ventricles, EVs have the opportunity to contact neural stem cell (NSC) niches. We purified EVZ310 produced by a choroid plexus-cell line, by differential centrifugation and flotation in iodixanol density gradients. We co-cultured suspensions of EVZ310 with NSC from the lateral and third ventricle. Alternatively, EVZ310 were dried-down on a polymer substrate and a suspension of NSCs was added. In both cases, EV Z310 induced, in a dose dependent manner, the NSC to rapidly form cellular networks accompanied by the expression of genes characteristic for neurons (Tuj1) and astrocytes (Gfap). NSCs from either origin responded to EVZ310 qualitatively and quantitatively in a very similar way. By contrast, EVMEF purified from mouse embryonic fibroblasts had little effect on both types of NSC. Mass spectrometry revealed significant differences in composition between EVMEF and EVZ310 proteomes. Notably, EVZ310 were enriched for the membrane or membrane associated proteins known to be involved in neurogenesis, cell differentiation, membrane trafficking and membrane organization.
HostingRepository	PRIDE
AnnounceDate	2022-12-18
AnnouncementXML	Submission_2022-12-18_10:28:55.661.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	IvanSilbern
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-24 00:57:57	ID requested
⏵ 1	2022-12-18 10:28:56	announced
2	2023-11-14 08:55:07	announced	2023-11-14: Updated project metadata.

submitter keyword: cerebrospinal fluid, neural stem cells, mass spectrometry,Extracellular vesicles, exosome assay, neural stem cell differentiation, choroid plexus derived vesicle

Prof. Dr. HenningUrlaub
contact affiliation	Bioanalytical Mass Spectrometry Group, Max-Planck-Institute for Multidisciplinary Sciences, Göttingen, Germany; Institute for Clinical Chemistry, University Medical Center Göttingen, Göttingen, Germany
contact email	henning.urlaub@mpinat.mpg.de
lab head
IvanSilbern
contact affiliation	MPI Biophysical Chemistry
contact email	ivansilbern@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031862
     1. Label: PRIDE project
     2. Name: Extracellular vesicles derived from the choroid plexus trigger neural stem cells differentiation