PXD031845 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cell Surface Engineering Enables Surfaceome Profiling |
Description | Understanding which proteins are presented at the cell surface is essential for ongoing therapeutic development, and to expand our basic understanding of biology. We envisioned the integration of cell surface engineering with radical-mediated protein biotinylation to profile cell surface proteins (CSPs). This method relies on the pre-functionalization of cells with cholesterol lipid groups, followed by their conjugation with an APEX2 ascorbate peroxidase enzyme. The APEX2 enzyme allows for the covalent conjugation of biotin moieties to nearby residues for subsequent enrichment, and serves as a tool for the enrichment and subsequent MS analysis of CSPs. |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-06 |
AnnouncementXML | Submission_2022-05-06_09:16:44.196.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Zak Vilen |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-02-23 05:36:37 | ID requested | |
⏵ 1 | 2022-05-06 09:16:44 | announced | |
Publication List
Vilen Z, Reeves AE, O'Leary TR, Joeh E, Kamasawa N, Huang ML, Cell Surface Engineering Enables Surfaceome Profiling. ACS Chem Biol, 18(4):701-710(2023) [pubmed] |
Keyword List
submitter keyword: Surfaceome, PDAC, Proximity Labeling |
Contact List
Mia Lace Huang |
contact affiliation | Molecular Medicine, Huang Lab, Scripps Research, United States |
contact email | miahuang@scripps.edu |
lab head | |
Zak Vilen |
contact affiliation | Scripps Research |
contact email | Zvilen@scripps.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD031845 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD031845
- Label: PRIDE project
- Name: Cell Surface Engineering Enables Surfaceome Profiling