PXD031807-1

PXD031807 is an original dataset announced via ProteomeXchange.

Title	S-nitrosylation of p53 in A375 cells
Description	Nitric oxide (NO) is a pleiotropic signaling molecule that affects numerous biological functions. One unique posttranslational modification of proteins by NO is termed S-nitrosylation (SNO), but the role in modulating key proteins’ function in melanoma has not been fully characterized. In the present study, by conducting the biotin switch assay and mass spectrometry, we confirmed that p53 was S-nitrosylated under nitrosative stress. Further proteomic characterization showed that Cys242, Cys275, and Cys277 were S-nitrosylated in A375 cells treated with 100 µM GSNO.
HostingRepository	PRIDE
AnnounceDate	2025-07-21
AnnouncementXML	Submission_2025-07-20_16:03:58.061.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yong Qin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-21 05:48:30	ID requested
⏵ 1	2025-07-20 16:03:58	announced

10.3390/ijms26136512;

Grigoruta M, Li L, Chen L, Meng F, Rosenblatt KP, Li Y, Grimm EA, Qin Y, S-Nitrosylation of p53 in Melanoma Cells Under Nitrosative Stress. Int J Mol Sci, 26(13):(2025) [pubmed]

submitter keyword: A375, mass spectrometry,S-nitrosylation, p53, melanoma cells

Yong Qin
contact affiliation	The University of Texas at El Paso
contact email	yqin@utep.edu
lab head
Yong Qin
contact affiliation	The University of Texas at El Paso
contact email	yqin@utep.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD031807

PRIDE project URI

• PRIDE
  1. PXD031807
     1. Label: PRIDE project
     2. Name: S-nitrosylation of p53 in A375 cells