PXD031790-1

PXD031790 is an original dataset announced via ProteomeXchange.

Title	Double-stranded RNA viruses are released from Trichomonas vaginalis inside exosomes and modulate the exosomal cargo.
Description	Trichomonas vaginalis is a parasitic protist that infects the human urogenital tract. During the infection, trichomonads adhere to the host mucosa, acquire nutrients from the vaginal/prostate environment, and release small extracellular vesicles (sEVs) that contribute to the trichomonad adherenceand modulate the host-parasite communication. Approximately 40% - 70% of T. vaginalis strains harbor double-stranded RNA virus called Trichomonasvirus (TVV). Naked TVV particles have the potential to stimulate a proinflammatory response in human cells, however, the mode of TVV release from trichomonads to the environment is not clear. In this report, we showed for the first time that TVV particles are released from T. vaginalis cells within sEVs. The sEVs loaded with TVV stimulated a higher proinflammatory response of human HaCaT cells in comparison to sEVs from TVV negative parasites. Moreover, a comparison of T. vaginalis isogenic TVV plus and TVV minus clones revealed a significant impact of TVV infection on the sEV proteome and RNA cargo. Small EVs from TVV positive trichomonads contained 12 enriched and 8 unique proteins including membrane-associated BspA adhesine, and about a 2.5-fold increase in the content of small regulatory tsRNA. As T. vaginalis isolates are frequently infected with TVV, the release of TVV via sEVs to the environment represents an important factor with the potential to enhance inflammation-related pathogenesis during trichomoniasis.
HostingRepository	PRIDE
AnnounceDate	2022-04-22
AnnouncementXML	Submission_2022-04-22_09:34:18.238.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD031790
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Karel Harant
SpeciesList	scientific name: Trichomonas vaginalis; NCBI TaxID: 5722;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-21 03:33:16	ID requested
⏵ 1	2022-04-22 09:34:18	announced

Dataset with its publication pending

submitter keyword: Trichomonas vaginalis , RNA virus

Jan Tachezy
contact affiliation	Department of Parasitology, Faculty of Science, Charles University, BIOCEV, Průmyslová 595, 25242 Vestec, Czech Republic
contact email	tachezy@natur.cuni.cz
lab head
Karel Harant
contact affiliation	Charles University
contact email	harant@natur.cuni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD031790

PRIDE project URI

• PRIDE
  1. PXD031790
     1. Label: PRIDE project
     2. Name: Double-stranded RNA viruses are released from Trichomonas vaginalis inside exosomes and modulate the exosomal cargo.