PXD031788-2

PXD031788 is an original dataset announced via ProteomeXchange.

Title	A time-resolved multi-omics atlas of Acanthamoeba castellanii encystment
Description	Encystment is a common response of most protists to stress. Although some signaling pathways regulating this process were reported, our knowledge remains limited. Here, we mapped the molecular changes occurring in the amoeba Acanthamoeba castellanii during the early steps of encystment. De novo analysis of the transcriptome identified more than 150,000 previously undescribed transcripts that were utilized for mass spectrometry-based proteomics analysis after triggering starvation. With this strategy, we identified thousands of protein sequences absent from the reference genome. These results revealed the regulation of expected biological processes, such as the shutdown of cell proliferation at transcript level followed by down-regulation of proteins. The phosphoproteome showed a quick regulation of phosphorylation sites involved in cytoskeleton remodeling and translation regulation, among other processes underlying A. castellanii encystment. To our knowledge, this work constitutes the first time-resolved molecular atlas of an encysting organism and a useful resource to further investigating amoebae encystment.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:55:06.774.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Magalie Duchateau
SpeciesList	scientific name: Acanthamoeba castellanii str. Neff; NCBI TaxID: 1257118;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-21 03:32:25	ID requested
1	2022-07-12 03:37:28	announced
⏵ 2	2023-11-14 08:55:07	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Acanthamoeba castellanii,Encystment, time-resolved molecular atlas

Ascel SAMBA LOUAKA
contact affiliation	Laboratoire Ecologie et Biologie des Interactions, UMR CNRS 7267 Université de Poitiers ; UFR SFA; Pole Biologie Sante Bât. B36/B37 ; 1 Rue Georges Bonnet ; TSA 51106 86073 POITIERS Cedex 9, FRANCE
contact email	ascel.samba@univ-poitiers.fr
lab head
Magalie Duchateau
contact affiliation	Institut Pasteur
contact email	magalie.duchateau@pasteur.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD031788

PRIDE project URI

• PRIDE
  1. PXD031788
     1. Label: PRIDE project
     2. Name: A time-resolved multi-omics atlas of Acanthamoeba castellanii encystment