PXD031783-1

PXD031783 is an original dataset announced via ProteomeXchange.

Title	HDX-MS of the human eEF1B complex
Description	Protein synthesis in eukaryotic cell is spatially and structurally compartmentalized that ensures high efficiency of this process. One of the distinctive features of higher eukaryotes is the existence of stable multi-protein complexes involved in translation. Here we present the data on human guanine-nucleotide exchange factor (GEF) complex, eEF1B, comprising α, β and γ subunits. The project concerns HDX-MS measurements of individual subunits of this complex contributing to understanding of their spatial organization. In addition, we present HDX-MS data on binary complexes of the subunits and on the eEF1B complex as a whole, helping to position the subunits inside the complex.
HostingRepository	PRIDE
AnnounceDate	2022-08-02
AnnouncementXML	Submission_2022-08-02_02:57:26.143.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michał Kistowski
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-21 03:08:46	ID requested
⏵ 1	2022-08-02 02:57:26	announced
2	2023-11-14 08:55:54	announced	2023-11-14: Updated project metadata.

10.1093/NAR/GKAC685;

submitter keyword: Human eEF1B, Protein samples, HDX-MS

Prof. Michał Dadlez
contact affiliation	Mass Spectrometry Laboratory, Institute of Biochemistry and Biophysics PAS
contact email	michald@ibb.waw.pl
lab head
Michał Kistowski
contact affiliation	Institute of Biochemistry and Biophysics PAS
contact email	mkisto@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD031783

PRIDE project URI

• PRIDE
  1. PXD031783
     1. Label: PRIDE project
     2. Name: HDX-MS of the human eEF1B complex