PXD031774-1

PXD031774 is an original dataset announced via ProteomeXchange.

Title	A proteomic landscape of Candida albicans in the stepwise evolution to fluconazole resistance
Description	As an opportunistic fungal pathogen, Candida albicans is a major cause of superficial and systemic infections in immunocompromised patients. The increasing rate of azole resistance in C. albicans has brought further challenges to clinical therapy. In this study, we collect five isogenic C. albicans strains recovered over discrete intervals from an HIV-infected patient who suffered a two-year recurrent oropharyngeal candidiasis. Azole resistance was known from the clinical history to have developed gradually in this patient, and this was confirmed by MIC assay of each strain. Proteomic techniques can be used to investigate more comprehensively how resistance develops in pathogenic fungi over time. Our study is the first using tandem mass tag (TMT) labeling combined with liquid chromatography-mass spectrometry (LC-MS/MS) technology to investigate the acquired resistance mechanisms of serial C. albicans isolates at the proteomic level. A total of 4029 proteins have been identified, of which 3766 have been quantified. Compared with Ca1, bioinformatics analysis showed that differentially expressed proteins were mainly associated with the following aspects: down-regulation of glycolysis/gluconeogenesis, pyruvate metabolism, fatty acid degradation and oxidative stress response proteins in all four subsequent strains, but – remarkably – activation of amino acids metabolism in Ca8 and Ca14; increase in the protection against osmotic stress or excessive copper toxicity, up-regulation of respiratory chain activity, and suppression of iron transport in Ca17. By tracing proteomic alterations in this set of isogenic resistance isolates, we acquire mechanistic insight into the steps involved in the acquisition of azole resistance in C. albicans.
HostingRepository	PRIDE
AnnounceDate	2022-02-23
AnnouncementXML	Submission_2022-02-23_06:17:16.404.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nana Song
SpeciesList	scientific name: Candida albicans (Yeast); NCBI TaxID: 5476;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-21 02:44:24	ID requested
⏵ 1	2022-02-23 06:17:16	announced

Dataset with its publication pending

submitter keyword: Candida albicans, quantitative proteomics, acquired azole resistance

Xiaofang Li
contact affiliation	Department of Medical Mycology, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College
contact email	lxf3568@163.com
lab head
Nana Song
contact affiliation	Department of Medical Mycology, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College
contact email	nanas25@foxmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031774
     1. Label: PRIDE project
     2. Name: A proteomic landscape of Candida albicans in the stepwise evolution to fluconazole resistance