PXD031772 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Enrichment of NICD4 interacting proteins by three different methods. |
Description | There are several methods to bring down the bait and interactors from the cell lysates, each with its own advantages and disadvantages. Recently, the most commonly used method is one step AP, TAP and proximity-labeling. In order to analyze the Strengths and weaknesses of the three methods, fusion proteins were constructed by NICD4 with FLAG, SFB (S tag-2x Flag tag-SBP tag) and TurboID respectively, NICD4 and its interactors were obtained by purification. By analyzing these proteins, we believe that SFB-TAP is the most reliable and effective method to identify interacting proteins. |
HostingRepository | PRIDE |
AnnounceDate | 2022-08-12 |
AnnouncementXML | Submission_2022-08-12_00:00:11.506.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD031772 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | bian weixiang |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-02-21 02:43:07 | ID requested | |
⏵ 1 | 2022-08-12 00:00:12 | announced | |
Publication List
Bian W, Jiang H, Feng S, Chen J, Wang W, Li X, Protocol for establishing a protein-protein interaction network using tandem affinity purification followed by mass spectrometry in mammalian cells. STAR Protoc, 3(3):101569(2022) [pubmed] |
Keyword List
submitter keyword: NICD4, SFB-TAP, FLAG tag AP, TurboID. |
Contact List
Xu Li |
contact affiliation | School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China |
contact email | lixu@westlake.edu.cn |
lab head | |
bian weixiang |
contact affiliation | Westlake University |
contact email | bianweixiang@westlake.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD031772 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD031772
- Label: PRIDE project
- Name: Enrichment of NICD4 interacting proteins by three different methods.