PXD031749-2

PXD031749 is an original dataset announced via ProteomeXchange.

Title	Lipid mediated–phase separation of AGO proteins on the ER controls nascent peptide ubiquitination
Description	we sought to determine whether dmAGO1 and Ltn1 share common ubiquitinated nascent peptide targets. For this purpose, we performed the UBA bead-based immunoprecipitation followed by mass spec experiments to identify the ubiquitinated nascent peptide targets of dmAGO1 and Ltn1, respectively. In this assay, we used four different samples including gfp knockdown, vcp knockdown, vcp-ago1 double knockdown and vcp-ltn1 double knockdown S2 cells.
HostingRepository	iProX
AnnounceDate	2022-02-18
AnnouncementXML	Submission_2023-12-24_20:39:10.681.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yajie Gao
SpeciesList	scientific name: Drosophila melanogaster; NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-17 22:42:17	ID requested
1	2022-02-17 22:42:58	announced
⏵ 2	2023-12-24 20:39:11	announced	2023-12-25: Update information.

Gao Y, Zhu Y, Wang H, Cheng Y, Zhao D, Sun Q, Chen D, Lipid-mediated phase separation of AGO proteins on the ER controls nascent-peptide ubiquitination. Mol Cell, 82(7):1313-1328.e8(2022) [pubmed]

submitter keyword: AGO1, ER, Nascent peptide, Drosophila, Lipidbinding

Dahua Chen
contact affiliation	Institute of Biomedical Research, Yunnan University
contact email	chendh@ioz.ac.cn
lab head
Yajie Gao
contact affiliation	Institute of Biomedical Research, Yunnan University
contact email	gaoyajie@ynu.edu.cn
dataset submitter

iProX dataset URI