<<< Full experiment listing


PXD031748 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFunctional interactomes of the Ebola virus polymerase identified by proximity proteomics in the context of viral replication
DescriptionEbola virus (EBOV) critically depends on the viral polymerase to replicate and transcribe the viral RNA genome in the cytoplasm of host cells, where cellular factors can antagonize or facilitate the virus life cycle. Here we leveraged proximity proteomics and conducted an siRNA screen to define the functional interactome of EBOV polymerase. As proof-of-principle, we validated two cellular mRNA decay factors from 35 identified host factors: eukaryotic peptide chain release factor subunit 3a (eRF3a/GSPT1) and up-frameshift protein 1 (UPF1). Our data suggest that EBOV can subvert restrictions of cellular mRNA decay and repurpose both GSPT1 and UPF1 to promote viral replication. Treating EBOV-infected human hepatocytes with a drug candidate that targets GSPT1 for degradation significantly reduced viral RNA load and particle production. Our work demonstrates the utility of proximity proteomics to capture the functional host-interactome of the EBOV polymerase and to illuminate host-dependent regulations of viral RNA synthesis.
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterGogce Ceren Crynen
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Ebolavirus; NCBI TaxID: 186536;
ModificationListBiotin; TMT6plex; Deamidated; Oxidation
Instrumentinstrument model
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-02-17 11:57:10ID requested
12022-03-21 11:39:24announced
Publication List
no publication
Keyword List
submitter keyword: Ebola virus, biotinylated, EBOV, split-TurboID
Contact List
Erica Ollmann Saphire
contact affiliationLa Jolla Institute for Immunology
contact emailerica@lji.org
lab head
Gogce Ceren Crynen
contact affiliationthe scripps research institute
contact emailgcrynen@scripps.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000088859/