PXD031748-1
PXD031748 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Functional interactomes of the Ebola virus polymerase identified by proximity proteomics in the context of viral replication |
| Description | Ebola virus (EBOV) critically depends on the viral polymerase to replicate and transcribe the viral RNA genome in the cytoplasm of host cells, where cellular factors can antagonize or facilitate the virus life cycle. Here we leveraged proximity proteomics and conducted an siRNA screen to define the functional interactome of EBOV polymerase. As proof-of-principle, we validated two cellular mRNA decay factors from 35 identified host factors: eukaryotic peptide chain release factor subunit 3a (eRF3a/GSPT1) and up-frameshift protein 1 (UPF1). Our data suggest that EBOV can subvert restrictions of cellular mRNA decay and repurpose both GSPT1 and UPF1 to promote viral replication. Treating EBOV-infected human hepatocytes with a drug candidate that targets GSPT1 for degradation significantly reduced viral RNA load and particle production. Our work demonstrates the utility of proximity proteomics to capture the functional host-interactome of the EBOV polymerase and to illuminate host-dependent regulations of viral RNA synthesis. |
| HostingRepository | MassIVE |
| AnnounceDate | 2022-03-21 |
| AnnouncementXML | Submission_2022-03-21_11:39:24.432.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Gogce Ceren Crynen |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Ebolavirus; NCBI TaxID: 186536; |
| ModificationList | Biotin; TMT6plex; Deamidated; Oxidation |
| Instrument | instrument model |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-02-17 11:57:10 | ID requested | |
| ⏵ 1 | 2022-03-21 11:39:24 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Ebola virus, biotinylated, EBOV, split-TurboID |
Contact List
| Erica Ollmann Saphire | |
|---|---|
| contact affiliation | La Jolla Institute for Immunology |
| contact email | erica@lji.org |
| lab head | |
| Gogce Ceren Crynen | |
| contact affiliation | the scripps research institute |
| contact email | gcrynen@scripps.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000088859/ |
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