PXD031733-1

PXD031733 is an original dataset announced via ProteomeXchange.

Title	Thermophilic Geobacillus WSUCF1 secretome for saccharification of AFEX and EA pretreated corn stover
Description	It is difficult to identify individual hydrolyzing enzymes using routine enzyme assays in the laboratory, which only give a collective picture of the secretome enzymatic activity. A proteomic analysis method based on liquid chromatography followed by tandem mass spectrometry (LCMS/MS) has proven significant for profiling CAZymes in secretome and is a powerful tool to identify enzymes that are otherwise impossible to detect. Furthermore, proteomic analysis of secretome against genomic data using different bioinformatics tools provides more details about CAZymes and relative quantifications of enzymes in each sample.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-11_23:56:08.843.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD031733
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Mengchuan Zhuang
SpeciesList	scientific name: Geobacillus sp. WSUCF1; NCBI TaxID: 886559;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ FT

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-17 03:59:47	ID requested
⏵ 1	2022-08-11 23:56:09	announced

Bhalla A, Arce J, Ubanwa B, Singh G, Sani RK, Balan V, WSUCF1 Secretome for Saccharification of Ammonia Fiber Expansion and Extractive Ammonia Pretreated Corn Stover. Front Microbiol, 13():844287(2022) [pubmed]

submitter keyword: LC-MSMS, Geobacillus, ThermoFisher LTQ-FT Ultra Mass Spectrometer

Venkatesh Balan
contact affiliation	Department of Engineering Technology, College of Technology, University of Houston, TX 77479, USA
contact email	vbalan@UH.EDU
lab head
Mengchuan Zhuang
contact affiliation	University of Houston, College of Technology
contact email	mzhuang2@uh.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD031733

PRIDE project URI

• PRIDE
  1. PXD031733
     1. Label: PRIDE project
     2. Name: Thermophilic Geobacillus WSUCF1 secretome for saccharification of AFEX and EA pretreated corn stover