PXD031728-1

PXD031728 is an original dataset announced via ProteomeXchange.

Title	Interspecies isobaric labeling-based proteomics reveals protein changes in the ovary of Aedes aegypti co-infected with ZIKV and Wolbachia
Description	Zika is a vector-borne disease caused by an arbovirus (ZIKV) and overwhelmingly transmitted by Ae. aegypti. This disease is linked to adverse fetal outcomes, mostly microcephaly in newborns, and other clinical aspects such as acute febrile illness and neurologic complications, for example Guillain-Barré syndrome. One of the actual most promising strategies to mitigate arbovirus transmission involves releasing Ae. aegypti mosquitoes carrying the maternally inherited endosymbiont bacteria Wolbachia pipientis. The presence of Wolbachia is associated with a reduced susceptibility to arboviruses and a fitness cost in mosquito life-history traits as fecundity and fertility. However, the mechanisms by which Wolbachia influences metabolic pathways leading to differences in egg production remains poorly known. In order to investigate the impact of co-infections on the reproductive tract of the mosquito, we applied a quantitative proteomic strategy based on an isobaric labeling to investigate the influence of Wolbachia wMel and ZIKV infection in Ae. aegypti ovaries. For what we know, this is the most complete proteome of Ae. aegypti ovaries reported so far, with a total of 3913 proteins identified. We were able to quantify a total of 1044 Wolbachia proteins in complex sample tissue of Ae. aegypti ovary employing mass spectrometry-based quantitative methods. Furthermore, we describe and discuss proteins and pathways altered in Ae. aegypti during ZIKV infections, Wolbachia infections, co-infection Wolbachia/ZIKV, and compared with no infection, focusing on immune and reproductive aspects of Ae. aegypti.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-12_00:05:39.335.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michele Martins
SpeciesList	scientific name: Aedes aegypti; NCBI TaxID: 7159;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-17 03:44:11	ID requested
⏵ 1	2022-08-12 00:05:40	announced

submitter keyword: Proteome

Quantitative

Aedes aegypti

Wolbachia

Zika virus

Juvenile Hormone

immune defense.

Magno Junqueira
contact affiliation	Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil.
contact email	magnojunqueira@iq.ufrj.br
lab head
Michele Martins
contact affiliation	Federal University of Rio de Janeiro
contact email	martinsmichele.94@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031728
     1. Label: PRIDE project
     2. Name: Interspecies isobaric labeling-based proteomics reveals protein changes in the ovary of Aedes aegypti co-infected with ZIKV and Wolbachia