PXD031718-3

PXD031718 is an original dataset announced via ProteomeXchange.

Title	Global proteomic alterations in extracellular vesicles induced by oncogenic PIK3CA mutations
Description	MCF10A cells, and two isogenic mutant cell lines with known mutations in PIK3CA (E545 K and H1047R) were cultured in triplicate using culture medium depleted of extracellular vesicles. Cell culture supernatants were collected to study the extracellular vesicles (EVs) released by these cells. EVs were isolated from the samples by ultracentrifugation and their proteins digested. The resulting peptides were labeled with TMT and analyzed by mass spectrometry to identify differentially expressed proteins.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:06:48.368.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Akhilesh Pandey
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-16 08:14:13	ID requested
1	2023-10-22 12:03:37	announced
2	2023-11-14 09:06:15	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:06:49	announced	2024-10-22: Updated project metadata.

10.1002/pmic.202200077;

Saraswat M, Garapati K, Kim J, Budhraja R, Pandey A, Proteomic alterations in extracellular vesicles induced by oncogenic PIK3CA mutations. Proteomics, 22(19-20):e2200077(2022) [pubmed]

submitter keyword: MCF10A cells,Extracellular vesicles, Proteomics, PIK3CA

Akhilesh Pandey
contact affiliation	Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota 55905, United States
contact email	pandey.akhilesh@mayo.edu
lab head
Akhilesh Pandey
contact affiliation	Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905
contact email	pandey.akhilesh@mayo.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD031718

PRIDE project URI

• PRIDE
  1. PXD031718
     1. Label: PRIDE project
     2. Name: Global proteomic alterations in extracellular vesicles induced by oncogenic PIK3CA mutations