PXD031655-1
PXD031655 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Quantitiave analysis of protein methylome reveals the cellular response to ionizing radiation |
| Description | Protein methylation plays important roles in DNA damage signaling. To date, there is still a lack of global profiling of whole-cell methylation changes during the DNA damage response and repair. In this study, using HILIC affinity enrichment combined with MS analysis, we conducted a quantitative analysis of the methylated proteins in HEK293T cells in response to IR-induced DNA damage. In total, 235 distinct methylation sites responding to IR treatment were identified, and 38% of them were previously unknown. Multiple RNA-binding proteins were differentially methylated upon DNA damage stress. Furthermore, we identified 14 novel methylations in DNA damage response-related proteins. Moreover, we validated the function of PARP1 K23 methylation in repairing IR-induced DNA lesions. |
| HostingRepository | iProX |
| AnnounceDate | 2022-02-14 |
| AnnouncementXML | Submission_2022-06-21_23:50:35.860.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Chenxi Jia |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-02-13 21:11:24 | ID requested | |
| ⏵ 1 | 2022-06-21 23:50:36 | announced | |
| 2 | 2023-12-24 20:37:56 | announced | 2023-12-25: Update information. |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Protein methylation, DNA damage response, HILIC-affinity enrichment |
Contact List
| Yali Chen | |
|---|---|
| contact affiliation | State Key Laboratory of Proteomics, National Center for Protein Sciences – Beijing, Beijing Proteome Research Center, Beijing Institute of Lifeomics, Beijing 102206, China |
| contact email | cyali_2000@163.com |
| lab head | |
| Chenxi Jia | |
| contact affiliation | Beijing Proteome Research Center/ National Center for Protein Sciences(Beijing) |
| contact email | cjia99@126.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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