PXD031643-1

PXD031643 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Age induced interactome remodeling in muscle mitochondria
Description	A novel quantitative crosslinking mass spectrometry technique (qXL-MS) was applied to elucidate interactome changes in aged murine skeletal muscle mitochondria that contribute to age-related mitochondrial functional decline. Reported here are results from initial investigations of murine muscle mitochondrial interactomes that enable identification of statistically significant changes associated with aging. Newly developed isobaric quantitative protein interaction reporter (iqPIR) technologies15 enabled reproducible detection of age-related mitochondrial interactome changes. Muscle mitochondria from young and old mice were isolated, cross-linked with iqPIR molecules, young and old mitochondrial samples were paired, process and analyzed to quantify age-related mitochondrial interactome changes. In parallel, mitochondria were also subjected to additional measurements of mitochondrial protein yield, functional measurements including oxygen consumption rates on Complex I and Complex II substrates, and citrate synthase activity.
HostingRepository	PRIDE
AnnounceDate	2024-05-23
AnnouncementXML	Submission_2024-05-22_23:48:55.275.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anna Bakhtina
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-13 13:24:44	ID requested
⏵ 1	2024-05-22 23:48:56	announced
2	2024-10-22 06:41:33	announced	2024-10-22: Updated project metadata.

Publication List

10.1038/s43587-023-00366-5;
Bakhtina AA, Pharaoh GA, Campbell MD, Keller A, Stuppard RS, Marcinek DJ, Bruce JE, Skeletal muscle mitochondrial interactome remodeling is linked to functional decline in aged female mice. Nat Aging, 3(3):313-326(2023) [pubmed]

10.1038/s43587-023-00366-5;

Bakhtina AA, Pharaoh GA, Campbell MD, Keller A, Stuppard RS, Marcinek DJ, Bruce JE, Skeletal muscle mitochondrial interactome remodeling is linked to functional decline in aged female mice. Nat Aging, 3(3):313-326(2023) [pubmed]

Keyword List

submitter keyword: proteomics, protein interactions,Murine muscle, mitochondria, XL-MS

submitter keyword: proteomics, protein interactions,Murine muscle, mitochondria, XL-MS

Contact List

James E. Bruce
contact affiliation	Department of Genome Sciences, University of Washington
contact email	jimbruce@uw.edu
lab head
Anna Bakhtina
contact affiliation	University of Washington
contact email	anna.bakhtina91@gmail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD031643
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD031643

PRIDE project URI

Repository Record List

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