PXD031638-2

PXD031638 is an original dataset announced via ProteomeXchange.

Title	Evolutionary quantitative proteomics of reproductive interactions in Drosophila
Description	We present data using a novel method to simultaneously identify and quantify transferred male seminal proteins and the female reproductive proteome using multiplexed Tandem-Mass-Tag (TMT) isobaric labelling of the lower female reproductive tracts dissected from virgin- or recently mated- females of three species of the virilis group. We identified over 200 putative male ejaculate proteins many of which show differential abundance between species. We also identified over 2000 proteins providing the first description of the Drosophila female reproductive tract proteome outside of the melanogaster group which also shows significant divergence between species. We then assessed the utility of species-specific compared to single species query databases for protein identification and quantification.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:07:15.662.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Garlovsky
SpeciesList	scientific name: Drosophila americana; NCBI TaxID: 40366; scientific name: Drosophila virilis; NCBI TaxID: 7244; scientific name: Drosophila novamexicana; NCBI TaxID: 47314;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-13 12:58:50	ID requested
1	2023-10-24 11:10:27	announced
⏵ 2	2023-11-14 09:07:16	announced	2023-11-14: Updated project metadata.

Garlovsky MD, Ahmed-Braimah YH, Evolutionary Quantitative Proteomics of Reproductive Protein Divergence in Drosophila. Mol Cell Proteomics, 22(8):100610(2023) [pubmed]

submitter keyword: Drosophila novamexicana,Drosophila americana, TMT, sperm, Seminal fluid proteins, Female reproductive tract, reproductive isolation, postmating prezygotic isolation, Drosophila virilis

Yasir H. Ahmed-Braimah
contact affiliation	Department of Biology, Syracuse University, Syracuse NY, 13244, USA
contact email	yahmed@syr.edu
lab head
Martin Garlovsky
contact affiliation	Syracuse University
contact email	martingarlovsky@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD031638

PRIDE project URI

• PRIDE
  1. PXD031638
     1. Label: PRIDE project
     2. Name: Evolutionary quantitative proteomics of reproductive interactions in Drosophila