PXD031600-1

PXD031600 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of linear ubiquitination substrates based on NAEK-Ub probe in live cells
Description	While linear ubiquitin plays critical roles in multiple cell signaling pathways, few substrates have been identified. Global profiling of linear ubiquitin substrates represents a significant challenge because of the low endogenous level of linear ubiquitination, the none-specific binding of linear polyubiquitin chain, and the high background interferences arising from heterogeneous ubiquitin linkages (e.g. K48-, K63-). We developed a bioorthogonal linear ubiquitin probe by site-specific encoding of a norbornene amino acid (NAEK) on ubiquitin. This probe facilitates covalent labeling of linear ubiquitin substrates in live cells and enables selective enrichment and identification of linear-ubiquitin modified proteins. we applied this probe in the profiling of substrates of linear ubiquitination in HEK293T cells and GSCs.
HostingRepository	iProX
AnnounceDate	2022-02-11
AnnouncementXML	Submission_2023-04-19_22:03:40.761.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xianli Du
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-11 00:38:57	ID requested
⏵ 1	2023-04-19 22:03:41	announced

submitter keyword: Linear ubiquitination, bio-orthogonal probe, NAEK-Ub, live cells

Xiaotao Duan
contact affiliation	Beijing Institute of Pharmacology and Toxicology
contact email	duan_xt@hotmail.com
lab head
Xianli Du
contact affiliation	Beijing Institute of Pharmacology and Toxicology
contact email	dxlxp@163.com
dataset submitter

iProX dataset URI