PXD031600-1

PXD031600 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of linear ubiquitination substrates based on NAEK-Ub probe in live cells
Description	While linear ubiquitin plays critical roles in multiple cell signaling pathways, few substrates have been identified. Global profiling of linear ubiquitin substrates represents a significant challenge because of the low endogenous level of linear ubiquitination, the none-specific binding of linear polyubiquitin chain, and the high background interferences arising from heterogeneous ubiquitin linkages (e.g. K48-, K63-). We developed a bioorthogonal linear ubiquitin probe by site-specific encoding of a norbornene amino acid (NAEK) on ubiquitin. This probe facilitates covalent labeling of linear ubiquitin substrates in live cells and enables selective enrichment and identification of linear-ubiquitin modified proteins. we applied this probe in the profiling of substrates of linear ubiquitination in HEK293T cells and GSCs.
HostingRepository	iProX
AnnounceDate	2022-02-11
AnnouncementXML	Submission_2023-04-19_22:03:40.761.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xianli Du
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-11 00:38:57	ID requested
⏵ 1	2023-04-19 22:03:41	announced

Du X, Pang J, Gu B, Si T, Chang Y, Li T, Wu M, Wang Z, Wang Y, Feng J, Wu N, Man J, Li H, Li A, Zhang T, Wang B, Duan X, A bio-orthogonal linear ubiquitin probe identifies STAT3 as a direct substrate of OTULIN in glioblastoma. Nucleic Acids Res, 51(3):1050-1066(2023) [pubmed]

submitter keyword: Linear ubiquitination, bio-orthogonal probe, NAEK-Ub, live cells

Xiaotao Duan
contact affiliation	Beijing Institute of Pharmacology and Toxicology
contact email	duan_xt@hotmail.com
lab head
Xianli Du
contact affiliation	Beijing Institute of Pharmacology and Toxicology
contact email	dxlxp@163.com
dataset submitter

iProX dataset URI