PXD031591 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of Plin5 binding partners involved in lipid droplet-mitochondrial contacts |
Description | Perilipin 5 (Plin5) has been shown the induce contact between mitochondria and lipid droplets, though the mechanism by which this occurs is unknown. It has been shown that the unique C-terminal domain of Plin5 is responsible for inducing these contact sites. In this proteomics study, we identify binding partners which mediate this function by performing affinity purification-mass spectrometry using GFP-tagged Plin5 constructs where Plin5 is full length, lacking half of the c-terminal domain, or lacking the entire c-terminal domain. Of the several potential interactors, we validate Fatp4 as a direct interactor with the tether domain of Plin5. |
HostingRepository | PRIDE |
AnnounceDate | 2023-04-06 |
AnnouncementXML | Submission_2023-04-06_13:38:29.146.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | LauraHerring |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-02-10 15:13:50 | ID requested | |
⏵ 1 | 2023-04-06 13:38:29 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: metabolism, Plin5,AP-MS |
Contact List
SarahCohen |
contact affiliation | Department of Cell Biology and Physiology, UNC-Chapel Hill |
contact email | sarahcoh@ad.unc.edu |
lab head | |
LauraHerring |
contact affiliation | UNC-Chapel Hill |
contact email | laura_herring@med.unc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD031591
- Label: PRIDE project
- Name: Identification of Plin5 binding partners involved in lipid droplet-mitochondrial contacts