PXD031569-1

PXD031569 is an original dataset announced via ProteomeXchange.

Title	GPR56 (ADGRG1) and Transglutaminase (TG2) interaction in Regulation of Mesenchymal Transition in Glioblastoma.
Description	G protein-coupled receptor 56 (GPR56/ADGRG1) is an adhesion GPCR with an essential role in brain development and cancer. Elevated expression of GPR56 was observed in the clinical specimens of Glioblastoma (GBM), a highly invasive primary brain tumor. However, we found the expression to be variable across the specimens, presumably due to the intratumor heterogeneity of GBM. Therefore, we re-examined GPR56 expression in public domain spatial gene expression data and single-cell expression data for GBM, which revealed that GPR56 expression was high in cellular tumors, infiltrating tumor cells, and proliferating cells, low in microvascular proliferation and peri-necrotic areas of the tumor, especially in hypoxic mesenchymal-like cells. To gain a better understanding of the consequences of GPR56 downregulation in tumor cells and other molecular changes associated with it, we generated a sh-RNA-mediated control and GPR56 knockdown in the GBM cell line U373 and performed transcriptomics, proteomics, and phospho-proteomics analysis and using the data we propose a putative model to explain this functional and regulatory relationship of the two proteins.
HostingRepository	PRIDE
AnnounceDate	2022-05-04
AnnouncementXML	Submission_2022-05-04_04:16:05.311.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ravi Sirdeshmukh
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-10 13:05:35	ID requested
⏵ 1	2022-05-04 04:16:05	announced

Dataset with its publication pending

submitter keyword: GPR56, ADGRG1, TG2, GBM, mesenchymal

Dr. Ravi Sirdeshmukh
contact affiliation	Mazumdar Shaw Medical Center, Narayana Health, Bangalore, India
contact email	ravisirdeshmukh@gmail.com
lab head
Ravi Sirdeshmukh
contact affiliation	Institute of Bioinformatics
contact email	ravisirdeshmukh@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031569
     1. Label: PRIDE project
     2. Name: GPR56 (ADGRG1) and Transglutaminase (TG2) interaction in Regulation of Mesenchymal Transition in Glioblastoma.