PXD031568-1

PXD031568 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Cysteine residues are responsible for the sulfurous off-flavor formed in heated whey protein solutions
Description	Odor-active volatile sulfur compounds are formed in heated food protein systems. In the present study, hydrogen sulfide (H2S) was found to be the most abundant sulfur volatile in whey protein systems (whey protein isolate (WPI), a whey model system and single whey proteins) by Gas Chromatography-Flame Photometric Detector (GC-FPD) analysis after different heat treatments (60-90 C for 10 min, 90 C for 120 min and a UHT-like condition). Site-specific LC-MS/MS-based proteomic analysis was conducted to monitor desulfurization reactions in these protein systems to investigate the mechanism of H2S formation in heated WPI. H2S was detected in WPI after heating at 90 C for 10 min, and significantly increased at higher heat load (90 C for 120 min and the UHT treatment), which revealed the temperature- and time-dependence of heat-induced H2S generation in WPI. Cysteine (Cys) residues from beta-lactoglobulin were found to be responsible for the formation of H2S in heated WPI, presumably via beta-elimination.
HostingRepository	MassIVE
AnnounceDate	2022-02-10
AnnouncementXML	Submission_2022-02-10_02:42:09.442.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chengkang
SpeciesList	scientific name: Bos taurus; common name: cattle; NCBI TaxID: 9913;
ModificationList	Cys->Dha; unknown modification; Carbamidomethyl; unknown modification; unknown modification; unknown modification; unknown modification; unknown modification
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-10 02:24:38	ID requested
⏵ 1	2022-02-10 02:42:09	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Sulfur volatile, hydrogen sulfide, beta-lactoglobulin, beta-elimination, GC-FPD, LC-MS/MS

submitter keyword: Sulfur volatile, hydrogen sulfide, beta-lactoglobulin, beta-elimination, GC-FPD, LC-MS/MS

Contact List

Marianne Nissen Lund
contact affiliation	Department of Food Science, University of Copenhagen
contact email	mnl@food.ku.dk
lab head
Chengkang
contact affiliation	University of Copenhagen
contact email	licky_lck@hotmail.com
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000088811/