PXD031531-1

PXD031531 is an original dataset announced via ProteomeXchange.

Title	The PAF1 complex promotes 3‘ processing of pervasive transcripts
Description	The PAF1 complex (PAF1C) functions in multiple transcriptional processes involving RNA Polymerase II (Pol II). eRNAs and PROMPTs are pervasive transcripts transcribed by Pol II and rapidly degraded by the nuclear exosome complex after 3’ endonucleolytic cleavage by the Integrator complex (Integrator). Here we show that PAF1C has an unexpected role in the termination of eRNAs and PROMPTs that are cleaved 1-3 kb downstream of the transcription start site. Mechanistically, PAF1C facilitates recruitment of Integrator to sites of pervasive transcript cleavage, promoting timely cleavage and transcription termination. We also show that PAF1C recruits Integrator to coding genes, where PAF1C then dissociates from Integrator upon entry into processive elongation. Our results demonstrate an unexpected function for PAF1C in limiting the length and accumulation of pervasive transcripts that result from nonproductive transcription
HostingRepository	PRIDE
AnnounceDate	2022-03-03
AnnouncementXML	Submission_2022-03-02_19:31:27.677.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jing Han
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LCQ Classic; TripleTOF 5600; Q Exactive; 4700 Proteomics Analyzer; 4800 Proteomics Analyzer; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-09 05:14:19	ID requested
⏵ 1	2022-03-02 19:31:28	announced

Dataset with its publication pending

submitter keyword: Transcription regulation

enhancer RNA

3' End Processing

PAF1 complex

Integrator complex

pervasive transcription

Mo Chen
contact affiliation	Tsinghua university
contact email	mochen@mail.tsinghua.edu.cn
lab head
Jing Han
contact affiliation	Tsinghua University
contact email	hanj18@mails.tsinghua.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD031531

PRIDE project URI

• PRIDE
  1. PXD031531
     1. Label: PRIDE project
     2. Name: The PAF1 complex promotes 3‘ processing of pervasive transcripts