PXD031461-1

PXD031461 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics of airway epithelial cells from COPD and control pre- and post-influenza virus infection
Description	Chronic obstructive pulmonary disease (COPD) collectively refers to chronic and progressive lung diseases causing not fully reversible limitations in airflow. COPD patients are at high risk for severe respiratory symptoms upon influenza virus infection. Airway epithelial cells provide the first-line antiviral defense, but whether their susceptibility and response to influenza virus infection changes in COPD has not been elucidated. Therefore, this study aimed to i) compare the susceptibility of COPD- and control-derived airway epithelium to influenza virus, and ii) assess protein changes during influenza virus infection by quantitative proteomics. Fluorescent stainings confirmed expression of human- and avian-type influenza virus receptors in primary human bronchial epithelial cells (phBECs) from COPD patients (n=4) and controls (n=3) differentiated at the air-liquid interface. Subjects were closely matched in age, sex, and smoking history and, for COPD-derived phBECs, included stage II (n=2), stage III (n=1) and stage IV (n=1). Proteomics of fully differentiated phBECs pre- and post-influenza A virus infection with A/Puerto Rico/8/34 (PR8) revealed no significant differences between GOLD stage II/ III COPD (n=3) and control phBECs in terms of flu receptor expression, cell type composition, virus replication, or protein profile pre- and post-infection. In contrast, COPD GOLD stage IV phBECs (n=1) showed a distinct pattern of flu receptor expression and a typical COPD phenotype as assessed by a literature-derived panel of 20 proteins and quantification of cell type composition. Independent of health state, proteomics showed a robust antiviral response to influenza virus infection, as well as upregulation of several novel influenza virus-regulated proteins.
HostingRepository	PRIDE
AnnounceDate	2022-12-29
AnnouncementXML	Submission_2022-12-29_11:32:35.629.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JulianeMerl-Pham
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-05 13:47:07	ID requested
⏵ 1	2022-12-29 11:32:36	announced
2	2023-11-14 08:55:07	announced	2023-11-14: Updated project metadata.

submitter keyword: Chronic obstructive pulmonary disease (COPD), primary human bronchial epithelial cells, differentiated, proteomics, organotypic, influenza virus, flu receptor, airlift

ClaudiaStaab-Weijnitz
contact affiliation	Institute of Lung Health and Immunity and Comprehensive Pneumology Center with the CPC-M BioArchive, Helmholtz Zentrum München, Member of the German Center of Lung Research (DZL) - München (Germany)
contact email	staab-weijnitz@helmholtz-muenchen.de
lab head
JulianeMerl-Pham
contact affiliation	Research Unit Protein Science, Helmholtz Center Munich
contact email	juliane.merl@helmholtz-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/12/PXD031461

PRIDE project URI

• PRIDE
  1. PXD031461
     1. Label: PRIDE project
     2. Name: Quantitative proteomics of airway epithelial cells from COPD and control pre- and post-influenza virus infection