PXD031459-2

PXD031459 is an original dataset announced via ProteomeXchange.

Title	Glycan shield of the ebolavirus envelope glycoprotein GP
Description	The envelope glycoprotein GP of the ebolaviruses is essential for host cell attachment and entry. It is also the primary target of the protective and neutralizing antibody response in both natural infection and vaccination. GP is heavily glycosylated with up to 17 predicted N-linked sites, numerous O-linked glycans in its disordered mucin-like domain (MLD), and three predicted C-linked mannosylation sites. Glycosylation of GP is important for host cell attachment to cell-surface lectins, as well as GP stability and fusion activity. Moreover, it has been shown to shield GP from neutralizing activity of serum antibodies. Here, we use mass spectrometry-based glycoproteomics to profile the site-specific glycosylation patterns of ebolavirus GP, including N-, O-, and C-linked glycans.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:57:14.786.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joost Snijder
SpeciesList	scientific name: Bundibugyo ebolavirus; NCBI TaxID: NCBITaxon:565995; scientific name: Zaire ebolavirus; NCBI TaxID: NCBITaxon:186538;
ModificationList	glycosylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-05 13:43:46	ID requested
1	2022-08-02 06:57:44	announced
⏵ 2	2023-11-14 08:57:15	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: virus

ebola

glycosylation

Joost Snijder
contact affiliation	Utrecht University
contact email	j.snijder@uu.nl
lab head
Joost Snijder
contact affiliation	Utrecht University
contact email	joost.snijder@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031459
     1. Label: PRIDE project
     2. Name: Glycan shield of the ebolavirus envelope glycoprotein GP