PXD031434-1

PXD031434 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Quality assurance of hematopoietic stem cells by macrophages determines stem cell clonality
Description	There are no described quality assurance mechanisms for newly formed stem cells. We observed intimate interactions between macrophages and blood stem cells in zebrafish embryos. Stressed stem cells were marked by surface Calreticulin, which stimulates macrophage interaction as an eat me signal. Macrophage-stem cell interactions either lead to removal of cytoplasmic material and stem cell proliferation or resulted in complete stem cell engulfment. Calreticulin knock down or embryonic macrophage depletion reduced the number of stem cell clones into adulthood. Our work supports a model in which embryonic macrophages determine hematopoietic clonality by monitoring stem cell quality.
HostingRepository	MassIVE
AnnounceDate	2022-08-19
AnnouncementXML	Submission_2022-08-19_13:39:02.142.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Bogdan Budnik
SpeciesList	scientific name: Danio rerio; common name: zebrafish; NCBI TaxID: 7955;
ModificationList	Oxidation; Deamidated
Instrument	instrument model

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-04 04:38:51	ID requested
⏵ 1	2022-08-19 13:39:02	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: hematopoietic stem cells, macrophages, stem cell clonality

submitter keyword: hematopoietic stem cells, macrophages, stem cell clonality

Contact List

Leonard Zon
contact affiliation	Harvard University
contact email	zon@enders.tch.harvard.edu
lab head
Bogdan Budnik
contact affiliation	Harvard University
contact email	bbudnik@mcb.harvard.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000088780/