<<< Full experiment listing

PXD031412-1

PXD031412 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome dynamics during transition from exponential to stationary phase in yeast
DescriptionGlycolysis is the central pathway for sugar metabolism in most living organisms. The single celled eukaryote yeast is a widely used model organism for higher eukaryotes in which the regulation of glycolysis is widely studied. S. cerevisiae is one of the few eukaryotic organisms that can efficiently grow under both aerobic and anaerobic conditions. Furthermore, this yeast switches from proliferation into a resting phase when nutrients are exhausted. However, little is known about the proteome dynamics that takes place during this transition, particularly under anaerobic conditions. Moreover, like many other organisms including humans, the genome of S. cerevisiae contains duplications which result in the expression of so called ‘isoenzymes’ in central metabolic pathways including glycolysis. Interestingly, the role of those ‘isoenzymes’ remains elusive to date. Here we describe a large-scale quantitative proteome study combining shotgun experiments using a nano-LC coupled to a QE plus Orbitrap mass spectrometer (Thermo, Germany), to capture the proteome dynamics during transition from proliferation into stationary phase, under both aerobic and anaerobic growth. Furthermore, we explore the proteome dynamics of a mutant yeast where the glycolytic isoenzymes are deleted.
HostingRepositoryPRIDE
AnnounceDate2023-04-15
AnnouncementXMLSubmission_2023-04-15_04:14:18.790.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMartinPabst
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListS-carboxamidoethyl-L-cysteine; monohydroxylated residue; deaminated residue
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-02-03 08:07:20ID requested
12023-04-15 04:14:19announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: growth,Yeast, TMT quantification, Orbitrap QE plus, glycolysis
Contact List
MartinPabst
contact affiliationBiotechnology, Applied Sciences, Delft University of Technology
contact emailm.pabst@tudelft.nl
lab head
MartinPabst
contact affiliationTUD
contact emailm.pabst@tudelft.nl
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD031412
PRIDE project URI
Repository Record List
[ + ]