PXD031388-2

PXD031388 is an original dataset announced via ProteomeXchange.

Title	Ubiquitin-based pathway acts inside chloroplasts to regulate photosynthesis
Description	Photosynthesis is the energetic basis for most life on Earth, and in plants it operates inside double-membrane-bound organelles called chloroplasts. The photosynthetic apparatus comprises numerous proteins encoded by the nuclear and organellar genomes. Maintenance of this apparatus requires the action of internal chloroplast proteases, but a role for the nucleocytosolic ubiquitin-proteasome system (UPS) was not expected owing to the barrier presented by the double-membrane envelope. Here, we show that photosynthesis proteins (including those encoded internally by chloroplast genes) are ubiquitinated, and processed via the CHLORAD pathway: they are degraded by the 26S proteasome following CDC48-dependent retrotranslocation to the cytosol. This demonstrates that the reach of the UPS extends to the interior of endosymbiotically-derived chloroplasts, where it acts to regulate one of the most fundamental processes of life.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:55:44.445.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Honglin Chen
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-01 04:56:54	ID requested
1	2022-12-06 02:18:26	announced
⏵ 2	2023-11-14 08:55:44	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: ubiquitinome,chloroplast, organelle, CHLORAD, proteolysis, ubiquitin

Shabaz Mohammed
contact affiliation	Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, UK
contact email	shabaz.mohammed@chem.ox.ac.uk
lab head
Honglin Chen
contact affiliation	Department of Biochemistry, University of Oxford
contact email	honglin.chen@st-hughs.ox.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031388
     1. Label: PRIDE project
     2. Name: Ubiquitin-based pathway acts inside chloroplasts to regulate photosynthesis