PXD031376-1

PXD031376 is an original dataset announced via ProteomeXchange.

Title	Differential metabolic adaptations of brain cells to feeding a ketogenic diet in mice
Description	It is largely unknown how cells in the central nervous system (CNS) adapt their energy metabolism to nutritional challenges or disease. To unravel the molecular basis of these adaptations, we generated a comprehensive proteome dataset of all major CNS cell types. Magnetic bead sorting was used to acutely isolate neurons, astrocytes, oligodendrocytes, microglia, and endothelial cells from the cortex of individual young and adult mice challenged by feeding ketogenic diet or by neuroinflammatory disease. Label-free quantification of cell type-specific proteomes was performed by using an ion mobility-enhanced data-independent acquisition (DIA) workflow with alternating low and elevated energy (referred to as UDMSE). The combination of tissue metabolomic and expression analyses with cellular proteomic and transcriptomic analyses revealed that the strategies to manage the altered availability of energy metabolites differed between CNS cell types. Our data provide insights into key mechanisms of cell type-specific metabolic remodeling and a framework for the crosstalk between CNS cells in response to CNS challenges, which may have implications for the management of neurological diseases.
HostingRepository	PRIDE
AnnounceDate	2022-08-22
AnnouncementXML	Submission_2022-08-22_09:45:07.062.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olaf Jahn
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-01 03:30:09	ID requested
⏵ 1	2022-08-22 09:45:07	announced

Dataset with its publication pending

submitter keyword: Brain metabolism, label-free proteomics, mass spectrometry, metabolomics, transcriptomics, ketogenic diet, inflammatory demyelinating disease

Olaf Jahn
contact affiliation	Neuroproteomics Group Max Planck Institute for Multidisciplinary Sciences, City Campus Hermann-Rein-Str. 3 37075 Goettingen, Germany Tel: +49 551 20131-313 Fax: +49 551 20131-323 E-mail: jahn@mpinat.mpg.de
contact email	jahn@mpinat.mpg.de
lab head
Olaf Jahn
contact affiliation	Proteomics Group, Max-Planck-Institute of Experimental Medicine, Göttingen
contact email	jahn@em.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD031376

PRIDE project URI

• PRIDE
  1. PXD031376
     1. Label: PRIDE project
     2. Name: Differential metabolic adaptations of brain cells to feeding a ketogenic diet in mice