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PXD031327-1

PXD031327 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFe3+-NTA magnetic beads as an alternative to spin column-based phosphopeptide enrichment
DescriptionProtein phosphorylation is a central mechanism of cellular signal transduction in living organisms. Phosphoproteomic studies aim to systematically catalogue, characterize, and comprehend alterations in phosphorylation states across multiple cellular conditions and are often incorporated into global proteomics workflows. Previously, we found that spin column-based Fe3+-NTA enrichment integrated well with our workflow but it remained a bottleneck for methods that require higher throughput or a scale that is beyond the maximum capacity of these columns. Here, we compare our well-established spin column-based enrichment strategy with one encompassing magnetic beads. Our data show little difference in both the number and properties of the phosphopeptides identified when using either method. In all, we illustrate how scalable and automation-friendly magnetic Fe3+-NTA beads can seamlessly substitute spin column-based Fe3+-NTA for global phosphoproteome profiling.
HostingRepositoryPRIDE
AnnounceDate2022-09-02
AnnouncementXMLSubmission_2022-09-02_12:12:40.451.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJoao Paulo
SpeciesList scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Eclipse; Orbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-01-30 10:56:22ID requested
12022-09-02 12:12:41announced
Publication List
Liu X, Rossio V, Thakurta SG, Flora A, Foster L, Bomgarden RD, Gygi SP, Paulo JA, -NTA magnetic beads as an alternative to spin column-based phosphopeptide enrichment. J Proteomics, 260():104561(2022) [pubmed]
Keyword List
submitter keyword: automation, phosphoproteome, IMAC, phosphopeptide enrichment
Contact List
Joao A. Paulo
contact affiliationCell Biology Harvard Medical School Boston, MA 02115
contact emailjoao_paulo@post.harvard.edu
lab head
Joao Paulo
contact affiliationHarvard Medical School
contact emailjoao_paulo@post.harvard.edu
dataset submitter
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Dataset FTP location
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