PXD031327 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Fe3+-NTA magnetic beads as an alternative to spin column-based phosphopeptide enrichment |
Description | Protein phosphorylation is a central mechanism of cellular signal transduction in living organisms. Phosphoproteomic studies aim to systematically catalogue, characterize, and comprehend alterations in phosphorylation states across multiple cellular conditions and are often incorporated into global proteomics workflows. Previously, we found that spin column-based Fe3+-NTA enrichment integrated well with our workflow but it remained a bottleneck for methods that require higher throughput or a scale that is beyond the maximum capacity of these columns. Here, we compare our well-established spin column-based enrichment strategy with one encompassing magnetic beads. Our data show little difference in both the number and properties of the phosphopeptides identified when using either method. In all, we illustrate how scalable and automation-friendly magnetic Fe3+-NTA beads can seamlessly substitute spin column-based Fe3+-NTA for global phosphoproteome profiling. |
HostingRepository | PRIDE |
AnnounceDate | 2022-09-02 |
AnnouncementXML | Submission_2022-09-02_12:12:40.451.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Joao Paulo |
SpeciesList | scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Eclipse; Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-01-30 10:56:22 | ID requested | |
⏵ 1 | 2022-09-02 12:12:41 | announced | |
Publication List
Liu X, Rossio V, Thakurta SG, Flora A, Foster L, Bomgarden RD, Gygi SP, Paulo JA, -NTA magnetic beads as an alternative to spin column-based phosphopeptide enrichment. J Proteomics, 260():104561(2022) [pubmed] |
Keyword List
submitter keyword: automation, phosphoproteome, IMAC, phosphopeptide enrichment |
Contact List
Joao A. Paulo |
contact affiliation | Cell Biology Harvard Medical School Boston, MA 02115 |
contact email | joao_paulo@post.harvard.edu |
lab head | |
Joao Paulo |
contact affiliation | Harvard Medical School |
contact email | joao_paulo@post.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD031327
- Label: PRIDE project
- Name: Fe3+-NTA magnetic beads as an alternative to spin column-based phosphopeptide enrichment